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Role of glycoprotein 78 and cidec in hepatic steatosis
- Source :
- Molecular Medicine Reports
- Publication Year :
- 2017
- Publisher :
- Spandidos Publications, 2017.
-
Abstract
- Hepatic glycoprotein (gp78), a membrane-anchored E3 ubiquitin ligase, has been reported to be involved in regulating lipid and energy metabolism in animals, and cell death-inducing DFFA-like effector c (cidec) has emerged as an important regulator of metabolism, which has been implicated in the process of fat differentiation. Nonalcoholic fatty liver disease is a metabolic disorder associated with hepatic steatosis. In the present study, to investigate the role of gp78 and cidec in hepatic steatosis, an in vitro cell culture model of hepatic steatosis was established, using the AML12 mouse hepatocyte cell line to assess the protein expression of gp78. The results of Oil Red O staining, phase contrast microscopy and triglyceride content detection experiments indicated that the overexpression of gp78 induced lipid accumulation, whereas gp78-knockdown led to a reduction in lipid accumulation in the AML12 cells. The increased expression of gp78 was associated with steatosis. The expression of cidec was consistent with gp78, and the colocalization of gp78 and cidec was observed on the surface of lipid droplets using immunofluorescence analysis. Furthermore, an interaction between gp78 and cidec was detected using coimmunoprecipitation analysis, and this interaction promoted lipid accumulation. Based on these data, it was hypothesized that gp78 is a regulator of hepatic steatosis, and that it may be a putative molecular mediator in metabolic diseases.
- Subjects :
- 0301 basic medicine
Cancer Research
Cell
Biology
Biochemistry
Cell Line
cell death-inducing DFFA-like effector c
lipids
Mice
03 medical and health sciences
chemistry.chemical_compound
Lipid droplet
Nonalcoholic fatty liver disease
steatosis
Genetics
medicine
Animals
Oil Red O
Molecular Biology
glycoprotein 78
Proteins
Articles
Cell cycle
medicine.disease
Cell biology
Ubiquitin ligase
Fatty Liver
Receptors, Autocrine Motility Factor
030104 developmental biology
medicine.anatomical_structure
Oncology
chemistry
hepatic cell
Cell culture
Gene Knockdown Techniques
Hepatocytes
biology.protein
Cancer research
Molecular Medicine
Steatosis
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....e0a0695b7544d44bef7ccd174068e6bb