Analytical possibilities for the relative estimation of the antioxidative capacity of honey varieties harvested in different regions of Serbia

Two different approaches, spectroscopic and electrochemical, were applied for the rough determination of the antioxidative capacity of honey samples. Honey samples of diverse botanical origin were collected in different geographical regions of Serbia. The total phenolic content (TPC) was determined by the Folin-Ciocalteu method. Cyclic voltammograms on a glassy carbon electrode in KCl supporting electrolyte were used to check the electrode sensitivity to the presence of honey. In order to calculate the Trolox equivalent antioxidant capacity (TEAC) of the studied honey samples, cyclic voltammograms were recorded for the Trolox standard. The results were expressed as mu mol of Trolox equivalents per kg of sample (mu mol TE kg(-1)). Good correlations were observed between the cyclic voltammetry data and the TPC determined by the Folin-Ciocalteu method and the radical scavenging activity (RSA) determined using the DPPH center dot(1,1-diphenyl-2-picrylhydrazyl) radical test. Cyclic voltammetry appears to be a highly attractive alternative method for a rapid estimation of the antioxidative capacity of honeys. It was found that polyfloral honey samples had the highest, whereas acacia honey showed the lowest values of TPC. Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3343]