Back to Search
Start Over
Listeria monocytogenes Response to Propionate Is Differentially Modulated by Anaerobicity
- Source :
- Pathogens, Vol 7, Iss 3, p 60 (2018), Pathogens, Volume 7, Issue 3
- Publication Year :
- 2018
- Publisher :
- MDPI AG, 2018.
-
Abstract
- Propionate is a common food preservative and one of the major fermentation acids in the intestines. Therefore, exposure to propionate is frequent for foodborne pathogens and likely takes place under suboxic conditions. However, it is not clear whether the absence of oxygen affects how pathogens respond to propionate. Here, we investigated how propionate exposure affects Listeria monocytogenes growth and virulence factor production under aerobic or anaerobic conditions and showed that oxygen indeed plays a key role in modulating L. monocytogenes response to propionate. Under aerobic conditions, propionate supplementations had no effect on planktonic growth but resulted in decreased adherent growth. Under anaerobic conditions, propionate supplementations resulted in a pH-dependent inhibition of planktonic growth and increased adherent growth. Cultures grown with propionate accumulated higher levels of acetoin under aerobic conditions but lower levels of ethanol under both aerobic and anaerobic conditions. Metabolic perturbations by propionate were also evident by the increase in straight chain fatty acids. Finally, propionate supplementations resulted in increased listeriolyin O (LLO) production under anaerobic conditions but decreased LLO production under aerobic conditions. These results demonstrate for the first time that the presence or absence of oxygen plays a critical role in shaping L. monocytogenes responses to propionate.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Preservative
030106 microbiology
chemistry.chemical_element
adherent growth
lcsh:Medicine
medicine.disease_cause
Oxygen
Article
03 medical and health sciences
chemistry.chemical_compound
Listeria monocytogenes
medicine
Immunology and Allergy
Food science
Molecular Biology
chemistry.chemical_classification
General Immunology and Microbiology
Acetoin
lcsh:R
Listeriolysin O
membrane fatty acid composition
030104 developmental biology
Infectious Diseases
chemistry
Propionate
listeriolysin O
Fermentation
Anaerobic exercise
short chain fatty acids
Subjects
Details
- Language :
- English
- ISSN :
- 20760817
- Volume :
- 7
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Pathogens
- Accession number :
- edsair.doi.dedup.....e100196e29ae44d1cdbf6d216a298fa7