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Expression patterns of five polymorphic membrane proteins during the Chlamydia abortus developmental cycle
- Source :
- Wheelhouse, N, Sait, M, Wilson, K, Aitchison, K, McLean, K, Smith, D G E & Longbottom, D 2012, ' Expression patterns of five polymorphic membrane proteins during the Chlamydia abortus developmental cycle ', Veterinary Microbiology, vol. 160, no. 3-4, pp. 525-9 . https://doi.org/10.1016/j.vetmic.2012.06.017, Veterinary Microbiology
- Publication Year :
- 2012
- Publisher :
- Elsevier, 2012.
-
Abstract
- It has been suggested that polymorphic membrane proteins (Pmps) belonging to the Type V autotransporter protein family play an important role in the pathogenesis of Chlamydia abortus (C. abortus; formerly Chlamydophila abortus) infection. In a previous study we demonstrated the expression of all the pmps at the transcriptional level. The purpose of this study was to measure the number of Pmp positive inclusions throughout the C. abortus developmental cycle to investigate heterogeneity in expression patterns. McCoy cells were infected with C. abortus and analysed for Pmp expression over a 72h period by fluorescent immunocytochemistry. Pmp18D could be detected at all analysed time points, and could only be accurately quantified from 36hpi while Pmp10G positive inclusions could be visualised from 36hpi. Expression of Pmps 13G, 16G and 17G could only be visualised later in the cycle and within less than half of visualised inclusions. These results indicate that while expression of specific Pmps is constitutive (Pmp18D), the pattern of expression of other Pmps is more variable. This suggests that different members of the Pmp family may play different roles within the developmental cycle of the organism, with some (Pmps10G and 18D) having roles throughout the cycle, while the heterogeneity of expression of others may aid in antigenic variation.
- Subjects :
- Short Communication
QH301 Biology
Immunocytochemistry
Chlamydia abortus
Polymorphic membrane protein
Microbiology
Cell Line
Pathogenesis
03 medical and health sciences
Mice
Antigenic variation
Animals
Chlamydia
030304 developmental biology
0303 health sciences
General Veterinary
biology
030306 microbiology
Gene Expression Profiling
General Medicine
Gene Expression Regulation, Bacterial
Chlamydia Infections
biology.organism_classification
Molecular biology
veterinary(all)
Chlamydophila abortus
Gene expression profiling
Membrane protein
Cell culture
Autotransporter domain
570 Life sciences
Bacterial Outer Membrane Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 03781135 and 18732542
- Database :
- OpenAIRE
- Journal :
- Wheelhouse, N, Sait, M, Wilson, K, Aitchison, K, McLean, K, Smith, D G E & Longbottom, D 2012, ' Expression patterns of five polymorphic membrane proteins during the Chlamydia abortus developmental cycle ', Veterinary Microbiology, vol. 160, no. 3-4, pp. 525-9 . https://doi.org/10.1016/j.vetmic.2012.06.017, Veterinary Microbiology
- Accession number :
- edsair.doi.dedup.....e11d2a144ece783cfd3e950beb02cd20