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Elevation of Glutamine Level by Selenophosphate Synthetase 1 Knockdown Induces Megamitochondrial Formation in Drosophila Cells
- Source :
- Journal of Biological Chemistry. 284:32881-32894
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- Although selenophosphate synthetase 1 (SPS1/SelD) is an essential gene in Drosophila, its function has not been determined. To elucidate its intracellular role, we targeted the removal of SPS1/SelD mRNA in Drosophila SL2 cells using RNA interference technology that led to the formation of vacuole-like globular structures. Surprisingly, these structures were identified as megamitochondria, and only depolarized mitochondria developed into megamitochondria. The mRNA levels of l(2)01810 and glutamine synthetase 1 (GS1) were increased by SPS1/SelD knockdown. Blocking the expression of GS1 and l(2)01810 completely inhibited the formation of megamitochondria induced by loss of SPS1/SelD activity and decreased the intracellular levels of glutamine to those of control cells suggesting that the elevated level of glutamine is responsible for megamitochondrial formation. Overexpression of GS1 and l(2)01810 had a synergistic effect on the induction of megamitochondrial formation and on the synthesis of glutamine suggesting that l(2)01810 is involved in glutamine synthesis presumably by activating GS1. Our results indicate that, in Drosophila, SPS1/SelD regulates the intracellular glutamine by inhibiting GS1 and l(2)01810 expression and that elevated levels of glutamine lead to a nutritional stress that provides a signal for megamitochondrial formation.
- Subjects :
- Glutamine
Tetrazolium Salts
Mitochondrion
Biology
Biochemistry
Selenophosphate synthetase 1
Cell Line
Selenium
Adenosine Triphosphate
Glutamine synthetase
Animals
Humans
Molecular Biology
Gene knockdown
Microscopy, Confocal
Phosphotransferases
fungi
Megamitochondria
Cell Biology
Molecular biology
Recombinant Proteins
Mitochondria
Microscopy, Electron
Thiazoles
Metabolism and Bioenergetics
Phenotype
Cell culture
Drosophila
RNA Interference
Intracellular
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 284
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....e12c383ae6c36a0a7ee1086486de3f63