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The protective effects of salvianolic acid A against hepatic ischemia-reperfusion injury via inhibiting expression of toll-like receptor 4 in rats
- Source :
- Archives of Medical Science, Vol 15, Iss 6, Pp 1599-1607 (2019), Archives of Medical Science : AMS
- Publication Year :
- 2019
- Publisher :
- Termedia Publishing House, 2019.
-
Abstract
- Introduction Ischemia-reperfusion injury (IRI) is a serious complication of hepatectomy and liver transplantation. The aim of this study was to evaluate the protective effects of salvianolic acid-A (Sal-A) against IRI-induced hepatocellular injury. Material and methods Forty rats were randomly divided into the following four groups: (1) sham group, (2) IR group, (3) Sal-A(10) group and (4) Sal-A(20) group. After 90 min of ischemia and 6 h of reperfusion, serum alanine aminotransferease (ALT) and apartate aminotransferase (AST) levels were measured; the amounts of malondialdehyde (MDA) and superoxide dismutase (SOD) in the liver tissue were determined; the expression of Bcl-2 and caspase-3 was detected and the severity of apoptosis, inflammation and pathological alterations were evaluated. Also apoptosis and mRNA and protein levels of TLR4 (toll-like receptor 4) were tested. Results The serum aminotransferases, hepatic MDA concentration, and apoptotic cells in the IR group were significantly higher than in the sham group (p < 0.01), whereas the Sal-A group values were lower than in the IR group (p < 0.05). Compared with the IR group, the Sal-A groups had significantly higher Bcl-2 expression and downregulated cleaved caspase-3 expression in liver tissue. Moreover, increased mRNA and protein levels of TLR4 in IR rats and Sal-A could improve the increased mRNA and protein levels of TLR4. Conclusions Sal-A had a synergistically protective effect on the liver tissue against IRI that might be due to decreased oxidative stress, inflammation, hepatocellular apoptosis and include, at least in part, the regulation of TLR4.
- Subjects :
- medicine.medical_specialty
Experimental Research
medicine.medical_treatment
toll-like receptor 4
lcsh:Medicine
Inflammation
Liver transplantation
medicine.disease_cause
liver
ischemia-reperfusion
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
parasitic diseases
medicine
salvianolic acid-a
030212 general & internal medicine
biology
business.industry
lcsh:R
apoptosis
General Medicine
Malondialdehyde
medicine.disease
Endocrinology
chemistry
Apoptosis
inflammation
biology.protein
medicine.symptom
Hepatectomy
business
Reperfusion injury
Oxidative stress
Subjects
Details
- Language :
- English
- ISSN :
- 18969151 and 17341922
- Volume :
- 15
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Archives of Medical Science
- Accession number :
- edsair.doi.dedup.....e15b6c1e20e65c367add8ec1f0d66af4