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Signaling through CD43 regulates CD4 T-cell trafficking
- Source :
- Blood. 110(8)
- Publication Year :
- 2007
-
Abstract
- The mucin-like protein CD43 is excluded from the immune synapse, and regulates T-cell proliferation as well as T-cell migration. While the CD43 cytoplasmic domain is necessary for regulation of T-cell activation and proliferation, the mechanism via which CD43 regulates trafficking is not well defined. To investigate whether CD43 phosphorylation regulates its function in T cells, we used tandem mass spectrometry and identified Ser76 in murine CD43 as a previously unidentified site of basal phosphorylation. Interestingly, mutation of this single serine to alanine greatly diminishes T-cell trafficking to the lymph node, while CD43 exclusion and CD43-mediated regulation of T-cell proliferation remain intact. Furthermore, the CD43 extracellular domain was also required for T-cell trafficking, providing a hitherto unknown function for the extracellular domain, and suggesting that the extracellular domain may be required to transduce signals via the cytoplasmic domain. These data reveal a novel mechanism by which CD43 regulates T-cell function, and suggest that CD43 functions as a signaling molecule, sensing extracellular cues and transducing intracellular signals that modulate T-cell function.
- Subjects :
- CD4-Positive T-Lymphocytes
Immunology
Molecular Sequence Data
Fluorescent Antibody Technique
Biology
Biochemistry
Immunological synapse
Transduction (genetics)
Mice
immune system diseases
Cell Movement
Tandem Mass Spectrometry
Transduction, Genetic
hemic and lymphatic diseases
Extracellular
Animals
Humans
Amino Acid Sequence
Phosphorylation
Conserved Sequence
Immunobiology
Leukosialin
Sequence Homology, Amino Acid
hemic and immune systems
Cell Biology
Hematology
Flow Cytometry
Cell biology
Cytoplasm
Mutation
Electrophoresis, Polyacrylamide Gel
Signal transduction
Intracellular
Function (biology)
Signal Transduction
Subjects
Details
- ISSN :
- 00064971
- Volume :
- 110
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi.dedup.....e16ca17b585300608989649a93b783ba