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RecF, UvrD, RecX and RecN proteins suppress DNA degradation at DNA double-strand breaks in Escherichia coli
- Source :
- Biochimie
- Publication Year :
- 2018
- Publisher :
- Elsevier, 2018.
-
Abstract
- Double strand breaks (DSBs) in E. coli chromosome (such as those induced by gamma rays) are repaired by recombination repair, during which a certain amount of DNA gets degraded. We monitored DNA degradation in gamma-irradiated cells to assess processing of DSBs. DNA degradation in irradiated cells is regulated by RecA protein concentration and its affinity of ssDNA binding, as well as by exonucleases that trim 3′-terminated ss tails. Here we determined the effects of proteins that affect formation and stability of RecA nucleofilaments on DNA degradation and cell survival. RecF and UvrD suppressed DNA degradation through RecA protein function and SOS induction, while also improving gamma survival. RecF and UvrD function in one pathway. Acting along with RecF, RecX suppressed DNA degradation and stimulated gamma-survival, which also depends on RecA protein and SOS induction. Furthermore, we determined a role in DNA degradation of several proteins that participate in DSB repair. RecN was required for DNA repair and for degradation suppression, acting on the RecABCD pathway. Furthermore, we show that SSB protein overproduction did not affect DNA degradation. Inactivation of RecG and RuvABC, proteins that catalyze the postsynaptic phase of recombination repair of DSBs, also did not affect DNA degradation, suggesting that once formed, recombination intermediates are not subject to DNA degradation, and that the postsynaptic phase is an irreversible, single-round process, unlike the presynaptic phase, which is mostly repetitive.
- Subjects :
- 0301 basic medicine
DNA, Bacterial
DNA repair
Genetics, Evolution and Phylogenetics
DSB repair
DSB processing
genome stability
gamma irradiation
030106 microbiology
Gamma irradiation
medicine.disease_cause
DNA Helicase
Biochemistry
Microbiology
Single-stranded binding protein
03 medical and health sciences
chemistry.chemical_compound
RuvABC
Genome stability
Escherichia coli Protein
medicine
Escherichia coli
DNA Breaks, Double-Stranded
Biology
Rec A Recombinase
Microbial Viability
biology
Escherichia coli Proteins
Gamma Ray
DNA Helicases
Chromosome
General Medicine
Cell biology
Rec A Recombinases
030104 developmental biology
chemistry
Gamma Rays
biology.protein
bacteria
Homologous recombination
Recombination
DNA
Subjects
Details
- Language :
- Croatian
- Database :
- OpenAIRE
- Journal :
- Biochimie
- Accession number :
- edsair.doi.dedup.....e17ac8ac8b60a916641b5c4487e46005