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Liquid chromatography coupled with time-of-flight tandem mass spectrometry for comprehensive phenolic characterization of pomegranate fruit and flower extracts used as ingredients in botanical dietary supplements
- Source :
- J Sep Sci
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Pomegranate (Punica granatum L.) fruit and flower extracts, which are rich sources of bioactive phenolics, are widely utilized as ingredients in botanical dietary supplements. While the phenolic characterization of extracts of pomegranate fruit has been previously studied by liquid chromatography with tandem mass spectrometry, there is lack of similar data for pomegranate flowers. Herein, liquid chromatography with time-of-flight tandem mass spectrometry was utilized to comprehensively characterize the phenolics present in two pomegranate extracts, previously studied for their in vitro and in vivo biological effects, namely, a patented commercial pomegranate fruit extract (Pomella®), and a pomegranate flower extract. Seventy-one phenolics were characterized in the pomegranate fruit extract with the vast majority identified in the flower extract. However, only the pomegranate fruit extract contained tannin-glucuronides and two punicalagin isomers (a characteristic pomegranate phenolic) were identified in this extract while four were identified in the flower extract. The previously reported compounds, pomellatannin and punicatannins A/B, were identified as unique chemical markers in the pomegranate fruit and flower extracts, respectively. This study will aid in the quality control, authentication, and standardization of these botanical ingredients to evaluate their potential health benefits in future planned pre-clinical and clinical studies. Also, this is the first phenolic characterization of a pomegranate flower extract using liquid chromatography with tandem mass spectrometry.
- Subjects :
- Time Factors
Filtration and Separation
Flowers
Health benefits
Tandem mass spectrometry
01 natural sciences
Article
Analytical Chemistry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Chemical marker
Phenols
Tandem Mass Spectrometry
Punicalagin
Chromatography
biology
Plant Extracts
Chemistry
010401 analytical chemistry
food and beverages
biology.organism_classification
POMEGRANATE FRUIT EXTRACT
0104 chemical sciences
Fruit
030220 oncology & carcinogenesis
Punica
Dietary Supplements
Chromatography, Liquid
Subjects
Details
- ISSN :
- 16159306
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- Journal of Separation Science
- Accession number :
- edsair.doi.dedup.....e1b443d20642f85f61217b89ca5797ff