The Securing of a Polyethylene Tube prior to Intravascular Dye Injection in Anatomic Microcirculation Research: The Three-TieTechnique

A simple and reliable method for securing the cannulated polyethylene tube to the blood vessel wall prior to intravascular dye injection is described. The three-tie technique has been used in many vascular anatomic studies without any leakage of the injection fluid taking place around the tube.