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In vivo immobilized carbonic anhydrase and its effect on the enhancement of CO2 absorption rate
- Source :
- Journal of biotechnology 336 (2021): 41–49. doi:10.1016/j.jbiotec.2021.06.016, info:cnr-pdr/source/autori:Fabbricino S.; Del Prete S.; Russo M.E.; Capasso C.; Marzocchella A.; Salatino P./titolo:In vivo immobilized carbonic anhydrase and its effect on the enhancement of CO2 absorption rate/doi:10.1016%2Fj.jbiotec.2021.06.016/rivista:Journal of biotechnology/anno:2021/pagina_da:41/pagina_a:49/intervallo_pagine:41–49/volume:336
- Publication Year :
- 2021
-
Abstract
- Reactive absorption into aqueous solutions promoted by carbonic anhydrase (CA, E.C. 4.2.1.1.) has been often proposed as a post-combustion CO2 capture process. The state of the art reveals the need for efficient biocatalyst based on carbonic anhydrase that can be used to further develop CO2 capture and utilization technologies. The present study is focused on the use of a thermostable CA-based biocatalyst. The carbonic anhydrase SspCA, from the thermophilic bacterium Sulfurihydrogenibium yellowstonense, was in vivo immobilized as membrane-anchored protein (INPN-SspCA) on the outer membrane of Escherichia coli cells. The dispersed biocatalyst, made by cell membrane debris, was characterized in terms of its contribution to the enhancement of CO2 absorption in carbonate/bicarbonate alkaline buffer at operating conditions relevant for industrial CO2 capture processes. The amount of immobilized enzyme, estimated by SDS-PAGE, resulted in about 1 mg enzyme/g membrane debris. The apparent kinetics of the biocatalyst was characterized through CO2 absorption tests in a stirred cell lab-scale reactor assuming a pseudo-homogeneous behaviour of the biocatalyst. At 298 K, the assessed values of the second-order kinetic constant ranged between 0.176 and 0.555 L∙mg−1∙s−1. Reusability of the biocatalyst after 24 h showed the absence of free enzyme release in the alkaline solvent. Moreover, the equilibration of dispersed cell membrane debris against the alkaline buffer positively affected the performances of the heterogeneous biocatalyst. These results encourage further studies on the in vivo immobilized SspCA aimed at optimizing the enzyme loading on the cell membrane and the handling of the biocatalyst in the CO2 absorption reactors.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Absorption (pharmacology)
Immobilized enzyme
enzymatic rective absorption
Bicarbonate
Bioengineering
01 natural sciences
Applied Microbiology and Biotechnology
In vivo immobilization
03 medical and health sciences
chemistry.chemical_compound
010608 biotechnology
Carbonic anhydrase
capture
Carbonic Anhydrases
chemistry.chemical_classification
Aqueous solution
biology
Bacteria
Chemistry
General Medicine
Carbon Dioxide
equipment and supplies
Enzymes, Immobilized
Solvent
CO
co2 capture
030104 developmental biology
Enzyme
Membrane
Chemical engineering
biology.protein
Enzymatic reactive absorption
Biotechnology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of biotechnology 336 (2021): 41–49. doi:10.1016/j.jbiotec.2021.06.016, info:cnr-pdr/source/autori:Fabbricino S.; Del Prete S.; Russo M.E.; Capasso C.; Marzocchella A.; Salatino P./titolo:In vivo immobilized carbonic anhydrase and its effect on the enhancement of CO2 absorption rate/doi:10.1016%2Fj.jbiotec.2021.06.016/rivista:Journal of biotechnology/anno:2021/pagina_da:41/pagina_a:49/intervallo_pagine:41–49/volume:336
- Accession number :
- edsair.doi.dedup.....e1cca2e6f7ab42f45d36f55b82916131