Back to Search
Start Over
CD109 promotes the tumorigenic ability and metastatic motility of pancreatic ductal adenocarcinoma cells
- Source :
- Pancreatology. 20:493-500
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Background Accumulating evidence indicates that CD109, a glycosylphosphatidylinositol-anchored glycoprotein, is highly expressed in human epithelial carcinomas of multiple organs including the pancreas, but its functional role in carcinoma development has not yet been fully clarified. The aim of this study was to investigate the role of CD109 in the malignancy of pancreatic ductal adenocarcinoma (PDAC). Methods PDAC specimens of 145 cases were immunostained for CD109, and correlations between CD109 expression and clinicopathological conditions were analyzed. CD109 expression in PANC-1 cells, a PDAC-derived cell line, was decreased by siRNA or shRNA and its effect on the malignancy of PANC-1 cells was examined. Results Suppression of CD109 expression in PANC-1 cells resulted in reduction of in vitro cell motility and tumorigenicity in xenografts. Based on these results, we investigated the relationship between CD109 expression and metastasis of PDAC using tumor tissue specimens. Among 106 recurrent cases of 145 PDAC, there was a tendency for CD109-positive cases to be accompanied by distant metastasis. Conclusions CD109 plays a critical role in the promotion of tumorigenic ability and cellular motility relating to metastasis of PDAC cells.
- Subjects :
- Male
endocrine system diseases
Endocrinology, Diabetes and Metabolism
Motility
GPI-Linked Proteins
Malignancy
Metastasis
Small hairpin RNA
Mice
03 medical and health sciences
0302 clinical medicine
Antigens, CD
Cell Movement
Cell Line, Tumor
Pancreatic cancer
medicine
Carcinoma
Animals
Humans
Neoplasm Metastasis
RNA, Small Interfering
Wound Healing
Hepatology
business.industry
Gastroenterology
Middle Aged
medicine.disease
Xenograft Model Antitumor Assays
digestive system diseases
Neoplasm Proteins
Gene Expression Regulation, Neoplastic
Pancreatic Neoplasms
medicine.anatomical_structure
Cell culture
030220 oncology & carcinogenesis
Cancer research
Female
030211 gastroenterology & hepatology
Pancreas
business
Carcinoma, Pancreatic Ductal
Subjects
Details
- ISSN :
- 14243903
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Pancreatology
- Accession number :
- edsair.doi.dedup.....e1ce095718734e1bdce27c2d0024a281