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A highly pathogenic GI-19 lineage infectious bronchitis virus originated from multiple recombination events with broad tissue tropism
- Source :
- Virus Research
- Publication Year :
- 2020
- Publisher :
- Elsevier B.V., 2020.
-
Abstract
- Highlight • The GI-19 strain was shown to be the dominant IBV lineage worldwide. • Isolate I0305/19 belongs to GI-19 lineage. • Isolate I0305/19 emerged through recombination events. • Isolate I0305/19 is a highly nephropathogenic strain. • Isolate I0305/19 showed broad tissue tropism in infected chickens.<br />In the present study, an IBV strain I0305/19 was isolated from a diseased commercial broiler flock in 2019 in China with high morbidity and mortality. The isolate I0305/19 was clustered together with viruses in sublineage D of GI-19 lineage on the basis of the complete S1 sequence analysis. Isolate I0305/19 and other GI-19 viruses isolated in China have the amino acid sequence MIA at positions 110 -112 in the S protein. Further analysis based on the complete genomic sequence showed that the isolate emerged through at least four recombination events between GI-19 ck/CH/LJS/120848- and GI-13 4/91-like strains, in which the S gene was found to be similar to that of the GI-19 ck/CH/LJS/120848-like strain. Pathological assessment showed the isolate was a nephropathogenic IBV strain that caused high morbidity of 100% and mortality of 80% in 1-day-old specific-pathogen-free (SPF) chicks. The isolate I0305/19 exhibited broader tropisms in different tissues, including tracheas, lungs, bursa of Fabricius, spleen, liver, kidneys, proventriculus, small intestines, large intestines, cecum, and cecal tonsils. Furthermore, subpopulations of the challenge virus were found in tissues of infected chickens; this finding is important in understanding how the virulent IBV strains can potentially replicate and evolve to cause disease. This information is also valuable for understanding the mechanisms of replication and evolution of other coronaviruses such as the newly emerged SARS-CoV-2.
- Subjects :
- infectious bronchitis virus
Cancer Research
China
animal structures
Sequence analysis
Virulence
broader tissue tropism
Infectious bronchitis virus
Genome, Viral
Biology
Virus Replication
Virus
Article
03 medical and health sciences
multiple recombination events
Virology
GI-19 lineage
Animals
Bursa of Fabricius
Gene
Phylogeny
Poultry Diseases
030304 developmental biology
Recombination, Genetic
0303 health sciences
030306 microbiology
high pathogenicity
Specific Pathogen-Free Organisms
Viral Tropism
Infectious Diseases
Viral replication
Spike Glycoprotein, Coronavirus
Tissue tropism
Coronavirus Infections
Chickens
Subjects
Details
- Language :
- English
- ISSN :
- 18727492 and 01681702
- Database :
- OpenAIRE
- Journal :
- Virus Research
- Accession number :
- edsair.doi.dedup.....e1d91f5e732f3834698efe02bef7e34a