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FACS Identifies Unique Cocaine-Induced Gene Regulation in Selectively Activated Adult Striatal Neurons
- Source :
- The Journal of Neuroscience. 31:4251-4259
- Publication Year :
- 2011
- Publisher :
- Society for Neuroscience, 2011.
-
Abstract
- Numerous studies with the neural activity marker Fos indicate that cocaine activates only a small proportion of sparsely distributed striatal neurons. Until now, efficient methods were not available to assess neuroadaptations induced specifically within these activated neurons. We used fluorescence-activated cell sorting (FACS) to purify striatal neurons activated during cocaine-induced locomotion in naive and cocaine-sensitizedcfos-lacZtransgenic rats. Activated neurons were labeled with an antibody against β-galactosidase, the protein product of the lacZ gene. Cocaine induced a unique gene expression profile selectively in the small proportion of activated neurons that was not observed in the nonactivated majority of neurons. These genes included altered levels of the immediate early genesarc,fosB, andnr4a3, as well as genes involved in p38 MAPK signaling and cell-type specificity. We propose that this FACS method can be used to study molecular neuroadaptations in specific neurons encoding the behavioral effects of abused drugs and other learned behaviors.
- Subjects :
- Transgene
Gene Expression
Biology
Article
Flow cytometry
Rats, Sprague-Dawley
Cocaine
Gene expression
medicine
Animals
Genes, Immediate-Early
Gene
Neurons
Regulation of gene expression
Analysis of Variance
Arc (protein)
medicine.diagnostic_test
Reverse Transcriptase Polymerase Chain Reaction
General Neuroscience
Cell sorting
Flow Cytometry
Immunohistochemistry
Corpus Striatum
Rats
Gene Expression Regulation
nervous system
Female
Rats, Transgenic
Neuroscience
FOSB
Subjects
Details
- ISSN :
- 15292401 and 02706474
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- The Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....e215eb3d134114392bf979771560a0f1
- Full Text :
- https://doi.org/10.1523/jneurosci.6195-10.2011