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EGFR in colorectal cancer: more than a simple receptor
- Source :
- Annals of Oncology. 17:962-967
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- Background Advances in the understanding of tumor biology have led to the development of targeted therapies allowing progress in colorectal cancer treatment. One of the most promising targets is the epidermal growth factor receptor (EGFR). Method The presence and distribution of high- and low-affinity EGFR was investigated retrospectively in a group of 82 colorectal cancer samples (43 normal colon–colon cancer paired samples) using a specific ligand binding assay (Scatchard Analysis). Findings A large majority of tumor samples exhibited one class of high-affinity binding sites (78%). Eighteen cases (22%) exhibited both high- and low-affinity binding sites. A wide interpatient variability was observed for the site number, with physiologically-relevant high-affinity sites ranging from 7 to 310 fmol/mg protein in tumors and from 6 to 313 fmol/mg protein in normal mucosa. A significant positive correlation was demonstrated between tumor and normal mucosa for the high-affinity Kd values and for the number of high-affinity sites, suggesting a common regulation for both tumor and normal tissue. Interpretation These observations (i) could explain recently-reported clinically-active EGFR targeting in colorectal tumors apparently negative for EGFR, and (ii) may offer a plausible explanation for the link observed between toxicity in normal tissue (cutaneous rash) and clinical outcome of patients treated with anti-EGFR drugs. Present data extends our understanding of EGFR identity in colorectal cancer which could be useful in reconsidering the predictive tools for the identification of tumors putatively responsive to EGFR targeted therapy.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Colorectal cancer
medicine.medical_treatment
Targeted therapy
Growth factor receptor
medicine
Humans
Epidermal growth factor receptor
Intestinal Mucosa
Receptor
Aged
Neoplasm Staging
Retrospective Studies
Aged, 80 and over
biology
business.industry
Ligand binding assay
Mucous membrane
Cancer
Hematology
Middle Aged
medicine.disease
ErbB Receptors
Kinetics
medicine.anatomical_structure
Oncology
Colonic Neoplasms
Cancer research
biology.protein
Female
Colorectal Neoplasms
business
Subjects
Details
- ISSN :
- 09237534
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- Annals of Oncology
- Accession number :
- edsair.doi.dedup.....e2173ef961ec05bdece0699011c026b9
- Full Text :
- https://doi.org/10.1093/annonc/mdl037