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Two distinct trophectoderm lineage stem cells from human pluripotent stem cells
- Source :
- The Journal of Biological Chemistry
- Publication Year :
- 2021
- Publisher :
- American Society for Biochemistry and Molecular Biology, 2021.
-
Abstract
- The trophectoderm layer of the blastocyst-stage embryo is the precursor for all trophoblast cells in the placenta. Human trophoblast stem (TS) cells have emerged as an attractive tool for studies on early trophoblast development. However, the use of TS cell models is constrained by the limited genetic diversity of existing TS cell lines and restrictions on using human fetal tissue or embryos needed to generate additional lines. Here we report the derivation of two distinct stem cell types of the trophectoderm lineage from human pluripotent stem cells. Analogous to villous cytotrophoblasts in vivo, the first is a CDX2- stem cell comparable with placenta-derived TS cells—they both exhibit identical expression of key markers, are maintained in culture and differentiate under similar conditions, and share high transcriptome similarity. The second is a CDX2+ stem cell with distinct cell culture requirements, and differences in gene expression and differentiation, relative to CDX2- stem cells. Derivation of TS cells from pluripotent stem cells will significantly enable construction of in vitro models for normal and pathological placental development.
- Subjects :
- 0301 basic medicine
induced pluripotent stem cell
syncytiotrophoblast
Cell Culture Techniques
hESC, human embryonic stem cell
KSR, knockout serum replacement
Biochemistry
S1P, sphingosine-1 phosphate
Transcriptome
S1PR, S1P receptor
0302 clinical medicine
EVT, extravillous trophoblast
Pregnancy
hiPSC, human induced pluripotent stem cell
CDX2 Transcription Factor
CDX2
Induced pluripotent stem cell
reproductive and urinary physiology
0303 health sciences
DMEM, Dulbecco's modified Eagle's medium
TGFβ, transforming growth factor-beta
ROCK, RhoA associated kinase
Embryo
Cell Differentiation
differentiation
TSCM, trophoblast stem cell medium
Cell biology
Trophoblasts
medicine.anatomical_structure
embryonic structures
dhS1P, dihydrospingosine-1-phosphate
Female
Cytotrophoblasts
trophoblast stem cells
Stem cell
Research Article
Pluripotent Stem Cells
Cell type
placenta
CTB, cytotrophoblast
Biology
03 medical and health sciences
medicine
Humans
Cell Lineage
TS, trophoblast stem
STB, syncytiotrophoblast
Molecular Biology
Embryonic Stem Cells
030304 developmental biology
GO, gene ontology
EGF, epidermal growth factor
PCA, Principal component analysis
Cytotrophoblast
030102 biochemistry & molecular biology
Trophoblast
Cell Biology
Embryonic stem cell
trophoblast
embryonic stem cell
FGF, fibroblast growth factor
Culture Media
030104 developmental biology
hPSC, human pluripotent stem cell
Cell culture
BSA, bovine serum albumin
030217 neurology & neurosurgery
extravillous trophoblast
Subjects
Details
- Language :
- English
- ISSN :
- 1083351X and 00219258
- Volume :
- 296
- Database :
- OpenAIRE
- Journal :
- The Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....e218649239c12b2ad7937121815a16bc