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Protective effects of Corchorus olitorius and Butea monosperma against Arsenic induced aberrant methylation and mitochondrial DNA damage in wistar rat model
- Source :
- Toxicology Reports, Toxicology Reports, Vol 8, Iss, Pp 30-37 (2021)
- Publication Year :
- 2020
- Publisher :
- Elsevier, 2020.
-
Abstract
- Graphical abstract<br />Highlights • Arsenic exposure through food causes genotoxic effects in vivo. • Sub chronic exposure to Arsenic causes pre-cancerous changes. • C. olitorious & B. monosperma have significant nephro & hepato protective potential. • Protective effect of both plants species is evident in molecular level. • Potent use as readily available food supplement to minimize Arsenic toxicity.<br />Millions of people around the world are chronically exposed to Arsenic (As) through food and drinking water. Studies revealed that Arsenic is genotoxic and causes damage to DNA. In this study, we evaluated Corchorus olitorius and Butea monosperma for their alleviative properties against Arsenic induced genotoxicity in vivo using Wistar Rat model. Arsenic exposed rats were given C. olitorius leaf powder and B. monosperma flower powder as supplementation with normal food. Methylation status of p53 promoter was measured using Methylation Sensitive Restriction Endonuclease PCR (MSRE-PCR) assay and mitochondrial DNA (mtDNA) copy number as well as occurrence of a common deletion in mtDNA in liver and kidney tissue was determined through quantitative realtime PCR (qPCR). Arsenic exposed rats after supplementation showed relatively less severe effects of toxicity evident by significantly higher amount of (p
- Subjects :
- Mitochondrial DNA
Site-specific methylation
Gentoxicity
qPCR, Quantitative Polymerase Chain Reaction
Health, Toxicology and Mutagenesis
chemistry.chemical_element
mtDNA deletion
010501 environmental sciences
Biology
Toxicology
medicine.disease_cause
01 natural sciences
Arsenic toxicity
03 medical and health sciences
0302 clinical medicine
ASTAspartate, aminotransferase
RA1190-1270
medicine
Butea
HG-AAS, Hydride Generation - Atomic Absorption Spectroscopy
Arsenic
0105 earth and related environmental sciences
ComputingMethodologies_COMPUTERGRAPHICS
MSRE-PCR, Methylation sensitive restriction enzyme digestion Polymerase Chain Reaction
ALT, Alanine aminotransferase
Regular Article
Methylation
biology.organism_classification
Molecular biology
Real-time polymerase chain reaction
chemistry
Toxicology. Poisons
Toxicity
As, Arsenic
MtDNA, Mitochondrial DNA
030217 neurology & neurosurgery
Genotoxicity
ROS, Reactive oxygen species
Phytotherapy
Subjects
Details
- Language :
- English
- ISSN :
- 22147500
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Toxicology Reports
- Accession number :
- edsair.doi.dedup.....e21d5dd05889c3692e4855d624024d13