398 Effects of protein level and supplemental methionine in late-gestation on colostrum quality and passive immunity transfer in beef cattle

The objective of this study was to evaluate the influence of metabolizable protein (MP) level and supplemental rumen-protected methionine (RPM) on colostrum quality and passive immunity transfer from beef cows fed during late-gestation. One hundred and forty-seven pregnant Angus crossbred cows and heifers were randomly assigned to one of six dietary treatments based on a 3 x 2 factorial arrangement. Diets included feeding to 90%, 100% and 110% MP requirements (NRC, 2016), offered with (without) 9 g/d of RPM. Cattle were individually fed a partially mixed ration (60% haylage/40% straw for cows; 70% haylage/30% straw for heifers) with supplements top-dressed daily for approximately 56 d prior to calving. Immediately after calving, a colostrum sample was collected from each dam for analysis of milk components, total protein, and IgG concentrations. Two d post-calving, serum samples were collected from each calf for analysis of total protein and IgG concentrations. A refractometer was used to estimate total protein as a preliminary estimate of the transfer of maternal IgG and total IgG concentrations were later determined using Radial Immunodiffusion (RID). Data were analyzed as a completely randomized block design using Proc GLIMMIX in SAS. Level of MP, RPM, and their interaction did not influence concentrations of fat, protein, BHB or SCC in colostrum (P ≥ 0.12). Supplementation of RPM decreased concentrations of MUN (45.92 vs. 54.51 ±3.17 mg/dl; P = 0.04), but did not change IgG (14.66 vs. 15.90 ±0.87 g/dl; P = 0.29). While MP level during gestation did not affect total protein or IgG concentrations in calf serum (P ≥ 0.64), providing supplemental RPM decreased total protein (5.79 vs. 6.33 ±0.13 g/dl; P = 0.002) and IgG concentrations in calf serum (3.44 vs. 4.30 ±0.25 g/dl; P = 0.01). These results suggest that providing supplemental methionine for beef cows during gestation may alter colostrum quality and decrease serum protein and IgG in offspring.