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Molecular Characterization and Sex Distribution of Chemosensory Receptor Gene Family Based on Transcriptome Analysis of Scaeva pyrastri
- Source :
- PLoS ONE, PLoS ONE, Vol 11, Iss 5, p e0155323 (2016)
- Publication Year :
- 2015
-
Abstract
- Chemosensory receptors play key roles in insect behavior. Thus, genes encoding these receptors have great potential for use in integrated pest management. The hover fly Scaeva pyrastri (L.) is an important pollinating insect and a natural enemy of aphids, mainly distributed in the Palearctic and Nearctic regions. However, a systematic identification of their chemosensory receptor genes in the antennae has not been reported. In the present study, we assembled the antennal transcriptome of S. pyrastri by using Illumina sequencing technology. Analysis of the transcriptome data identified 60 candidate chemosensory genes, including 38 for odorant receptors (ORs), 16 for ionotropic receptors (IRs), and 6 for gustatory receptors (GRs). The numbers are similar to those of other Diptera species, suggesting that we were able to successfully identify S. pyrastri chemosensory genes. We analyzed the expression patterns of all genes by using reverse transcriptase PCR (RT-PCR), and found that some genes exhibited sex-biased or sex-specific expression. These candidate chemosensory genes and their tissue expression profiles provide information for further studies aimed at fully understanding the molecular basis behind chemoreception-related behaviors in S. pyrastri.
- Subjects :
- 0301 basic medicine
Male
Physiology
lcsh:Medicine
Genes, Insect
Bioinformatics
Biochemistry
Ion Channels
Transcriptome
Medicine and Health Sciences
Animal Anatomy
Receptor
lcsh:Science
Phylogeny
Genetics
Multidisciplinary
Physics
Drosophila Melanogaster
Phylogenetic Analysis
Animal Models
Genomics
Calliphora
Electrophysiology
Insects
Multigene Family
Physical Sciences
Insect Proteins
Female
Drosophila
Drosophila melanogaster
Transcriptome Analysis
Research Article
Signal Transduction
Transmembrane Receptors
Arthropoda
Sequence analysis
Materials Science
Biophysics
Neurophysiology
Biology
Real-Time Polymerase Chain Reaction
Research and Analysis Methods
03 medical and health sciences
Model Organisms
Gene family
Animals
Animal Physiology
Sex Distribution
Molecular Biology Techniques
Gene
Molecular Biology
Illumina dye sequencing
Materials by Attribute
Molecular Biology Assays and Analysis Techniques
Base Sequence
Sequence Analysis, RNA
Diptera
Gene Expression Profiling
lcsh:R
fungi
Organisms
Biology and Life Sciences
Proteins
Computational Biology
Molecular Sequence Annotation
Cell Biology
Ligand-Gated Ion Channels
biology.organism_classification
Genome Analysis
Invertebrates
Gene expression profiling
030104 developmental biology
Gene Ontology
Gene Expression Regulation
Odorants
lcsh:Q
Antennae (Animal Physiology)
Zoology
Neuroscience
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....e26d9382e61468c5b4cfe17e077ba285