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Silencing GTSE-1 expression inhibits proliferation and invasion of hepatocellular carcinoma cells

Authors :
Wentao Zhang
Lei Guo
Shumin Zhang
Ju-Bo Zhang
Chenhao Zhou
Bo Zhang
Wanyong Chen
Xiaoqiang Li
Qing-Hai Ye
Ning Ren
Source :
Cell Biology and Toxicology
Publisher :
Springer Nature

Abstract

G2 and S phase-expressed-1 (GTSE1) was recently reported to upregulate in several types of human cancer, based on negatively regulate p53 expression. However, its expression and functional roles in hepatocellular carcinoma (HCC) remain unknown. In this study, GTSE1 was observed to be highly expressed in HCC specimens and cell lines both at messenger RNA (mRNA) and protein levels. Furthermore, high GTSE1 expression was positively associated with tumor size, venous invasion, advanced tumor stage, and short overall survival. Moreover, we generated stable GTSE1 knockdown HCC cell lines to explore the effects of GTSE1 silencing on the growth and invasion of HCC in vitro. In determining the pathway through which GTSE1 regulated cell proliferation and invasion, GTSE1 silencing was found to inhibit AKT phosphorylation and downregulated cell cycle-related protein. In addition, GTSE1 downregulation decreased the growth of xenografts. In conclusion, these results indicated for the first time that overexpression of GTSE1 was involved in the progress of HCC, enhancing proliferation and promoting cell invasion in HCC cells. Electronic supplementary material The online version of this article (doi:10.1007/s10565-016-9327-z) contains supplementary material, which is available to authorized users.

Details

Language :
English
ISSN :
07422091
Volume :
32
Issue :
4
Database :
OpenAIRE
Journal :
Cell Biology and Toxicology
Accession number :
edsair.doi.dedup.....e2777713b9c400f3d89a93a6fe99796f
Full Text :
https://doi.org/10.1007/s10565-016-9327-z