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Partially purified actinomycetes compounds enhance the intracellular damages in multi-drug resistant P. aeruginosa and K. pneumoniae
- Source :
- Saudi Journal of Biological Sciences, Saudi Journal of Biological Sciences, Vol 28, Iss 11, Pp 6057-6062 (2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- Based on the excellent nutrient level, the current study was focused on isolation and anti-bacterial activity of the actinomycetes from marine mangrove soil samples. As result, 10 different strains of actinomycetes strains were identified on actinomycetes isolation agar plates. The identified strains were shown with white, clear, uncontaminated well matured spore producing ability. Based on the initial observation, the isolated colonies were actinomycetes. The partially extracted crude compound shown excellent anti-bacterial activity against P. aeruginosa and K. pneumoniae with 15 mm and 13 mm zone of inhibitions were observed at 500 μL concentrations. The minimum inhibition concentration result was also confirmed the 500 μL concentration against both the tested concentration with high inhibition rate. Then, the intracellular damages, decreased cell growth of the crude actinomycetes extract treated bacterial strains were clearly observed by confocal laser scanning electron microscope. The extracellular damages of bacterial cell wall and shape of the both the pathogens were clearly shown by scanning electron microscope. Therefore, all the results were clearly supported to the partially extracted crude compound and it has excellent anti-bacterial activity against tested multi drug resistant bacteria.
- Subjects :
- Multi drug resistant bacteria
Cell growth
Chemistry
QH301-705.5
Biomedical application
Scanning electron microscope
Intracellular damage
Bacterial cell structure
Microbiology
law.invention
Spore
Agar plate
law
Actinomycetes
Extracellular
Original Article
Marine mangrove soil
Electron microscope
Biology (General)
General Agricultural and Biological Sciences
Intracellular
Subjects
Details
- Language :
- English
- ISSN :
- 22137106 and 1319562X
- Volume :
- 28
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Saudi Journal of Biological Sciences
- Accession number :
- edsair.doi.dedup.....e2a3a8f0c420f2365f3232420db99dd0