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mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ
- Source :
- Molecular plant. 12(9)
- Publication Year :
- 2018
-
Abstract
- RNA polymerase transcriptional pausing represents a major checkpoint in transcription in bacteria and metazoans, but it is unknown whether this phenomenon occurs in plant organelles. Here, we report that transcriptional pausing occurs in chloroplasts. We found that mTERF5 specifically and positively regulates the transcription of chloroplast psbEFLJ in Arabidopsis thaliana that encodes four key subunits of photosystem II. We found that mTERF5 causes the plastid-encoded RNA polymerase (PEP) complex to pause at psbEFLJ by binding to the +30 to +51 region of double-stranded DNA. Moreover, we revealed that mTERF5 interacts with pTAC6, an essential subunit of the PEP complex, although pTAC6 is not involved in the transcriptional pausing at psbEFLJ. We showed that mTERF5 recruits additional pTAC6 to the transcriptionally paused region of psbEFLJ, and the recruited pTAC6 proteins could be assembled into the PEP complex to regulate psbEFLJ transcription. Taken together, our findings shed light on the role of transcriptional pausing in chloroplast transcription in plants.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Chloroplasts
Photosystem II
Protein subunit
Arabidopsis
Plant Science
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Transcription (biology)
RNA polymerase
Organelle
Molecular Biology
biology
Arabidopsis Proteins
food and beverages
biology.organism_classification
Cell biology
Chloroplast
030104 developmental biology
chemistry
DNA
Bacteria
010606 plant biology & botany
Transcription Factors
Subjects
Details
- ISSN :
- 17529867
- Volume :
- 12
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Molecular plant
- Accession number :
- edsair.doi.dedup.....e2b548d72423cc18ff151513928d4c9d