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Assessment of principal parabens used in cosmetics after their passage through human epidermis-dermis layers (ex-vivo study)
- Source :
- Experimental Dermatology, Experimental Dermatology, Wiley, 2007, 16 (10), pp.830-6. ⟨10.1111/j.1600-0625.2007.00625.x⟩
- Publication Year :
- 2007
- Publisher :
- HAL CCSD, 2007.
-
Abstract
- International audience; Concern is continuously raised about the safety of parabens which are present in most of the cosmetic preparations. In this investigation, methyl-, ethyl-, propyl- and butyl paraben (MP, EP, PP, BP), in a commercial cosmetic lotion, were deposited on human skin fragments, collected after surgical operations. Permeated parabens were determined after their passage through human epidermis-dermis layers, fixed on Franz diffusion cells. Bovine serum albumin (3%) was employed as receptor fluid. Then, parabens were assessed by liquid chromatography. The objective of this research was to determine the permeation of these molecules through human epidermis-dermis layers, and their possible passage to body tissues and/or accumulation in skin layers. Two groups of experiments were performed. In the first experimental group (G1), unique doses of the cosmetic were deposited on skin fragments fixed on Franz cells (n = 6), at time 0 h, followed with different withdrawn times of the receptor fluid at 12, 24 and 36 h. G1 results demonstrated that parabens penetration was influenced by their lipophilicity: more lipophilic the parabens were (BP > PP > EP > MP), less they crossed the skin layers (BP < PP < EP < MP). The second experimental group (G2) was constituted of three equal deposits on each Franz cell (n = 6) at different hour times 0, 12 and 24 h followed with three withdrawn times of the receptor fluid at 12, 24 and 36 h. The G2 results indicated that investigated parabens had significant increasing permeations in skin layers. This situation provokes the accumulation of these molecules which were considered by some authors as the cause of skin toxicities and carcinogenicity.
- Subjects :
- Male
Pathology
Time Factors
Human skin
MESH: Skin Absorption
Cosmetics
010501 environmental sciences
01 natural sciences
Biochemistry
Toxicology
chemistry.chemical_compound
Bovine serum albumin
media_common
Skin
0303 health sciences
biology
integumentary system
Chemistry
Nuclear Proteins
RNA-Binding Proteins
Dermis
MESH: Cosmetics
3. Good health
Paraben
DNA-Binding Proteins
Epidermis (zoology)
medicine.anatomical_structure
Lotion
MESH: Permeability
Lipophilicity
[SDV.IMM]Life Sciences [q-bio]/Immunology
Female
Adult
medicine.medical_specialty
[SDV.IMM] Life Sciences [q-bio]/Immunology
media_common.quotation_subject
Skin Absorption
Parabens
Breast Neoplasms
Dermatology
In Vitro Techniques
Permeability
03 medical and health sciences
MESH: Skin
medicine
Humans
Molecular Biology
030304 developmental biology
0105 earth and related environmental sciences
Homeodomain Proteins
Chromatography
MESH: Humans
MESH: Time Factors
MESH: Adult
Penetration (firestop)
MESH: Dermis
Surgery
biology.protein
MESH: Parabens
Epidermis
MESH: Epidermis
MESH: Female
Ex vivo
Subjects
Details
- Language :
- English
- ISSN :
- 09066705 and 16000625
- Database :
- OpenAIRE
- Journal :
- Experimental Dermatology, Experimental Dermatology, Wiley, 2007, 16 (10), pp.830-6. ⟨10.1111/j.1600-0625.2007.00625.x⟩
- Accession number :
- edsair.doi.dedup.....e2c6a5e9ebe287c34216eee0eb15d343