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Effect of calcium glucoheptonate on proliferation and osteogenesis of osteoblast-like cells in vitro
- Source :
- PLoS ONE, PLoS ONE, Vol 14, Iss 9, p e0222240 (2019)
- Publication Year :
- 2019
-
Abstract
- Calcium is the key macromineral having a role in skeletal structure and function, muscle contraction, and neurotransmission. Bone remodeling is maintained through a constant balance between calcium resorption and deposition. Calcium deficiency is resolved through calcium supplementation, and among the supplements, water-soluble organic molecules attracted great pharmaceutical interest. Calcium glucoheptonate is a highly water-soluble organic calcium salt having clinical use; however, detailed investigations on its biological effects are limited. We assessed the effects of calcium glucoheptonate on cell viability and proliferation of osteoblast-like MG-63 cells. Calcium uptake and mineralization were evaluated using Alizarin red staining of osteoblast-like MG-63 cells treated with calcium glucoheptonate. Expression of osteogenic markers were monitored by western blotting, immunofluorescence, and qRT-PCR assays. Increased proliferation and calcium uptake were observed in the MG-63 cells treated with calcium glucoheptonate. The treatment also increased the expression of osteopontin and osteogenic genes such as collagen-1, secreted protein acidic and cysteine rich (SPARC), and osteocalcin. Calcium glucoheptonate treatment did not exert any cytotoxicity on colorectal and renal epithelial cells, indicating the safety of the treatment. This is the first report with evidence for its beneficial effect for pharmaceutical use in addressing calcium deficiency conditions.
- Subjects :
- Physiology
Peptide Hormones
Alizarin Staining
Ossification
Biochemistry
Epithelium
Bone remodeling
0302 clinical medicine
Osteogenesis
Animal Cells
Calcium glucoheptonate
Group-Specific Staining
Medicine and Health Sciences
Enzyme assays
Osteonectin
Osteopontin
Colorimetric assays
Bioassays and physiological analysis
Cells, Cultured
Staining
0303 health sciences
Multidisciplinary
MTT assay
biology
Cell Staining
Osteoblast
Enzymes
medicine.anatomical_structure
030220 oncology & carcinogenesis
Osteocalcin
293T cells
Medicine
Cell lines
Bone Remodeling
Cellular Types
Anatomy
Biological cultures
medicine.drug
Research Article
medicine.medical_specialty
Cell Survival
Science
chemistry.chemical_element
Calcium
Research and Analysis Methods
Bone resorption
Collagen Type I
03 medical and health sciences
Internal medicine
Cell Line, Tumor
medicine
Humans
030304 developmental biology
Cell Proliferation
Calcium metabolism
Osteoblasts
Phosphatases
Sugar Acids
Biology and Life Sciences
Proteins
Epithelial Cells
Cell Biology
Hormones
Endocrinology
HEK293 Cells
Biological Tissue
chemistry
Specimen Preparation and Treatment
Biochemical analysis
biology.protein
Enzymology
Caco-2 Cells
Physiological Processes
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....e2fde4a145165c9c83d7c0eb1bc61435