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Urinary ADAM12 and MMP-9/NGAL Complex Detect the Presence of Gastric Cancer
- Source :
- Cancer Prevention Research. 8:240-248
- Publication Year :
- 2015
- Publisher :
- American Association for Cancer Research (AACR), 2015.
-
Abstract
- Although the early diagnosis of gastric cancer provides the opportunity for curative endoscopic resection, comprehensive screening endoscopy would be invasive and expensive. To date, there is a complete absence of clinically useful gastric cancer biomarkers. With the goal of discovering noninvasive biomarkers for the early diagnosis of gastric cancer, we have conducted a case–control study using urine samples from individuals with gastric cancer versus healthy control samples. Of the enrolled 106 patients from September, 2012 to April, 2013, a cohort of 70 patients composed of 35 patients with gastric cancer and 35 age- and sex-matched healthy controls was analyzed. The gastric cancer group was composed of stage IA of 62.9% (22/35). The urinary levels of MMP-9/NGAL complex (uMMP-9/NGAL) and ADAM12 (uADAM12) were significantly higher in the gastric cancer group compared with the healthy control group as determined by monospecific ELISAs (uMMP-9/NGAL: median, 85 pg/mL vs. 0 pg/mL; P = 0.020; uADAM12: median, 3.35 ng/mL vs. 1.44 ng/mL; P < 0.001). Multivariate analysis demonstrated that both uMMP-9/NGAL and uADAM12 were significant, independent diagnostic biomarkers for gastric cancer. Moreover, MMP-9/NGAL activity was significantly elevated as determined by gelatin zymography. The combination of uMMP-9/NGAL with uADAM12 distinguished between control samples and gastric cancer samples with an AUC of 0.825 (P < 0.001) in an ROC analysis. Significantly, immunohistochemical analyses demonstrated a high coexpression of MMP-9 and NGAL (P < 0.001) and high expression of ADAM12 (P < 0.001) in gastric cancer tissues compared with adjacent normal tissues (N = 35). In summary, uMMP-9/NGAL and uADAM12 are potential noninvasive biomarkers for gastric cancer, including early-stage disease. Cancer Prev Res; 8(3); 240–8. ©2015 AACR.
- Subjects :
- Adult
Male
Cancer Research
medicine.medical_specialty
Pathology
Urinary system
Protein Array Analysis
ADAM12 Protein
Enzyme-Linked Immunosorbent Assay
Urine
Adenocarcinoma
Gastroenterology
Immunoenzyme Techniques
Young Adult
Lipocalin-2
Stomach Neoplasms
Proto-Oncogene Proteins
Internal medicine
Biomarkers, Tumor
medicine
Humans
Prospective Studies
Stage (cooking)
Prospective cohort study
Aged
Neoplasm Staging
Aged, 80 and over
business.industry
Case-control study
Membrane Proteins
Cancer
Middle Aged
Prognosis
medicine.disease
Lipocalins
ADAM Proteins
Matrix Metalloproteinase 9
ROC Curve
Oncology
Case-Control Studies
Female
Cancer biomarkers
Neoplasm Grading
business
Acute-Phase Proteins
Follow-Up Studies
Subjects
Details
- ISSN :
- 19406215 and 19406207
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Cancer Prevention Research
- Accession number :
- edsair.doi.dedup.....e30395b5fe95e8d253c08c375b7c23d1
- Full Text :
- https://doi.org/10.1158/1940-6207.capr-14-0229