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Studies on Collagenase Inhibitors. IV. Inhibitors of Bacterial Collagenase in Coptidis Rhizoma
- Source :
- YAKUGAKU ZASSHI. 111:538-541
- Publication Year :
- 1991
- Publisher :
- Pharmaceutical Society of Japan, 1991.
-
Abstract
- A hot aqueous extract of Coptidis Rhizoma had an inhibitory effect on the bacterial collagenase from Clostridium histolyticum. Active principles were isolated by silica gel column chromatography from the CHCl3 extract. Consequently, two inhibitors obtained were identified with the chloride of berberine and coptisine. The concentrations of the berberine and coptisine in the assay mixture to give 50% inhibition (IC50) were 0.73 mM and 0.16 mM, respectively. The type of inhibition by coptisine chloride was shown to be a mixture type from Lineweaver-Burk plots. Tetrahydroberberine, a reduction product of berberine chloride, had no inhibitory effect. This result suggests that the quaternary nitrogen of the alkaloids plays an important role in inhibitory activity.
- Subjects :
- Pharmacology
Coptisine
Chromatography
Berberine
biology
Pharmaceutical Science
biology.organism_classification
Chloride
chemistry.chemical_compound
Microbial Collagenase
Column chromatography
chemistry
Clostridium histolyticum
Berberine Chloride
Collagenase
medicine
IC50
Drugs, Chinese Herbal
medicine.drug
Subjects
Details
- ISSN :
- 13475231 and 00316903
- Volume :
- 111
- Database :
- OpenAIRE
- Journal :
- YAKUGAKU ZASSHI
- Accession number :
- edsair.doi.dedup.....e309f0449038cb0415d4781db61cb486