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Screening for sugarcane yellow leaf virus in sorghum in Florida revealed its occurrence in mixed infections with sugarcane mosaic virus and a new marafivirus

Screening for sugarcane yellow leaf virus in sorghum in Florida revealed its occurrence in mixed infections with sugarcane mosaic virus and a new marafivirus

Authors :
Robert Beiriger
Lihua Tang
Wardatou Boukari
Philippe Rott
Muhammad Tahir
Martha Hincapie
Dimitre Mollov
Chunyan Wei
Eva Mulandesa
Samuel Grinstead
University of South Florida [Tampa] (USF)
USDA-ARS : Agricultural Research Service
Guangxi University [Nanning]
Bahauddin Zakariya University (BZU)
Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI)
Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro
Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Plant Health Institute of Montpellier (UMR PHIM)
Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro
Florida Sugar Cane League
Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)
Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Source :
Crop Protection, Crop Protection, 2021, 139, pp.105373. ⟨10.1016/j.cropro.2020.105373⟩, Crop Protection, Elsevier, 2021, 139, pp.105373. ⟨10.1016/j.cropro.2020.105373⟩
Publication Year :
2021
Publisher :
HAL CCSD, 2021.

Abstract

International audience; Sugarcane yellow leaf virus (SCYLV) is an aphid-transmitted virus for which Melanaphis sacchari is the main vector. Almost all sugarcane varieties grown in Florida are susceptible to SCYLV infection. In this study, we investigated the prevalence of SCYLV in accessions of Sorghum bicolor which is another natural host of this virus. Two field experiments, one in 2016 with 19 sorghum lines and the other in 2017 with 15 lines, were established at Belle Glade, FL. Stalks collected randomly in planted and ratoon crops were tested by tissue-blot immunoassay (TBIA) and reverse transcription-polymerase chain reaction (RT-PCR). Over the two-year period, 366 of 423 S. bicolor samples tested positive by TBIA but SCYLV was detected by RT-PCR in only 12 of 161 randomly selected subsamples. Full genome sequences of SCYLV, sugarcane mosaic virus (SCMV) and a new marafivirus were obtained by high-throughput sequencing (HTS) from three TBIA positive sorghum samples. HTS data for all three viruses were confirmed by RT-PCR. The SCMV isolates from S. bicolor appeared to be a new strain of this virus species. Positive reaction of S. bicolor by TBIA using SCYLV antibodies could not be systematically associated with plant infection by SCYLV or another virus. This suggested the occurrence of a non-specific serological reaction with an unknown S. bicolor antigen. SCMV and the new marafivirus were also detected in Sorghum almum, suggesting that this weed is a reservoir for S. bicolor-infecting viruses in Florida.

Details

Language :
English
ISSN :
02612194 and 18736904
Database :
OpenAIRE
Journal :
Crop Protection, Crop Protection, 2021, 139, pp.105373. ⟨10.1016/j.cropro.2020.105373⟩, Crop Protection, Elsevier, 2021, 139, pp.105373. ⟨10.1016/j.cropro.2020.105373⟩
Accession number :
edsair.doi.dedup.....e312267dccf448965fd48147085a436d
Full Text :
https://doi.org/10.1016/j.cropro.2020.105373⟩