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Tetraspanins CD9 and CD81 function to prevent the fusion of mononuclear phagocytes
- Source :
- The Journal of Cell Biology
- Publication Year :
- 2003
- Publisher :
- The Rockefeller University Press, 2003.
-
Abstract
- Tetraspanins CD9 and CD81 facilitate the fusion between gametes, myoblasts, or virus-infected cells. Here, we investigated the role of these tetraspanins in the fusion of mononuclear phagocytes. Expression of CD9 and CD81 and their complex formation with integrins were up-regulated when blood monocytes were cultured under normal conditions. Under fusogenic conditions in the presence of Con A, CD9 and CD81 up-regulation was inhibited, and their complex formation with integrins was down-regulated. Anti-CD9 and -CD81 antibodies, which were previously shown to inhibit the fusion of gametes, myoblasts, and virus-infected cells, unexpectedly promoted the fusion of monocytes and alveolar macrophages. However, these effects were not due to altered cell adhesion, aggregation, or cytokine production. When stimulated in vitro or in vivo, alveolar macrophages and bone marrow cells of CD9- and CD81-null mice formed larger numbers of multinucleated cells than those of wild-type mice. Finally, CD9/CD81 double-null mice spontaneously developed multinucleated giant cells in the lung and showed enhanced osteoclastogenesis in the bone. These results suggest that CD9 and CD81 coordinately prevent the fusion of mononuclear phagocytes.
- Subjects :
- Integrins
medicine.medical_treatment
viruses
Recombinant Fusion Proteins
Integrin
Down-Regulation
Osteoclasts
chemical and pharmacologic phenomena
Bone Marrow Cells
Membrane Fusion
Article
Antibodies
Monocytes
Tetraspanin 29
Tetraspanin 28
Mice
Multinucleate
Antigens, CD
medicine
Concanavalin A
Animals
Humans
Cell adhesion
integrins
osteoclasts
cell fusion
macrophages
multinucleated giant cells
Mice, Knockout
Phagocytes
Membrane Glycoproteins
biology
Macrophages
Cell Membrane
Lipid bilayer fusion
Membrane Proteins
Cell Biology
biochemical phenomena, metabolism, and nutrition
In vitro
biological factors
Cell biology
Up-Regulation
Cytokine
medicine.anatomical_structure
Giant cell
embryonic structures
biology.protein
Leukocytes, Mononuclear
Bone marrow
Subjects
Details
- Language :
- English
- ISSN :
- 15408140 and 00219525
- Volume :
- 161
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- The Journal of Cell Biology
- Accession number :
- edsair.doi.dedup.....e33b85eb9824d2a5db527d50cfcf5143