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The nuclear variant of bone morphogenetic protein 2 (nBMP2) is expressed in macrophages and alters calcium response
- Source :
- Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019), Scientific Reports
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group, 2019.
-
Abstract
- We previously identified a nuclear variant of bone morphogenetic protein 2 (BMP2), named nBMP2, that is translated from an alternative start codon. Decreased nuclear localization of nBMP2 in the nBmp2NLStm mouse model leads to muscular, neurological, and immune phenotypes—all of which are consistent with aberrant intracellular calcium (Ca2+) response. Ca2+ response in these mice, however, has yet to be measured directly. Because a prior study suggested impairment of macrophage function in nBmp2NLStm mutant mice, bone marrow derived (BMD) macrophages and splenic macrophages were isolated from wild type and nBmp2NLStm mutant mice. Immunocytochemistry revealed that nuclei of both BMD and splenic macrophages from wild type mice contain nBMP2, while the protein is decreased in nuclei of nBmp2NLStm mutant macrophages. Live-cell Ca2+ imaging and engulfment assays revealed that Ca2+ response and phagocytosis in response to bacterial supernatant are similar in BMD macrophages isolated from naïve (uninfected) nBmp2NLStm mutant mice and wild type mice, but are deficient in splenic macrophages isolated from mutant mice after secondary systemic infection with Staphylococcus aureus, suggesting progressive impairment as macrophages respond to infection. This direct evidence of impaired Ca2+ handling in nBMP2 mutant macrophages supports the hypothesis that nBMP2 plays a role in Ca2+ response.
- Subjects :
- 0301 basic medicine
Staphylococcus aureus
Phagocytosis
Immunocytochemistry
Mutant
Bone Morphogenetic Protein 2
Gene Expression
lcsh:Medicine
Mice, Transgenic
Bone morphogenetic protein 2
Article
Calcium in biology
Mice
03 medical and health sciences
0302 clinical medicine
Immune system
Animals
Macrophage
lcsh:Science
Cell Nucleus
Multidisciplinary
Chemistry
Macrophages
lcsh:R
Wild type
Nuclear Proteins
Staphylococcal Infections
Molecular biology
3. Good health
030104 developmental biology
Calcium
lcsh:Q
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....e360a1545895526f1ffb2f6efc29d896
- Full Text :
- https://doi.org/10.1038/s41598-018-37329-5