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Binding of ferredoxin to algal photosystem I involves a single binding site and is composed of two thermodynamically distinct events
- Source :
- Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1859:234-243
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Despite the impressive progress made in recent years in understanding the early steps in charge separation within the photosynthetic reaction centers, our knowledge of how ferredoxin (Fd) interacts with the acceptor side of photosystem I (PSI) is not as well developed. Fd accepts electrons after transiently docking to a binding site on the acceptor side of PSI. However, the exact location, as well as the stoichiometry, of this binding have been a matter of debate for more than two decades. Here, using Isothermal Titration Calorimetry (ITC) and purified components from wild type and mutant strains of the green algae Chlamydomonas reinhardtii we show that PSI has a single binding site for Fd, and that the association consists of two distinct binding events, each with a specific association constant.
- Subjects :
- 0301 basic medicine
Photosynthetic reaction centre
Light
Stereochemistry
Genetic Vectors
Biophysics
Gene Expression
Chlamydomonas reinhardtii
Photosystem I
Biochemistry
Electron Transport
03 medical and health sciences
Escherichia coli
Cloning, Molecular
Photosynthesis
Binding site
Ferredoxin
Binding Sites
Photosystem I Protein Complex
biology
Chemistry
Algal Proteins
Isothermal titration calorimetry
Cell Biology
biology.organism_classification
Acceptor
Recombinant Proteins
Molecular Docking Simulation
Kinetics
030104 developmental biology
Docking (molecular)
Mutagenesis, Site-Directed
Ferredoxins
Thermodynamics
Oxidation-Reduction
Protein Binding
Subjects
Details
- ISSN :
- 00052728
- Volume :
- 1859
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Bioenergetics
- Accession number :
- edsair.doi.dedup.....e36fbc08fc9d27c92651861723350eba