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The effect of TGFβ1 on thermogenic markers is dependent on the degree of adipocyte differentiation
- Source :
- Bioscience Reports
- Publication Year :
- 2020
- Publisher :
- Portland Press Ltd., 2020.
-
Abstract
- Transforming growth factor β (TGFβ) a multifunctional cytokine is known to regulate cell proliferation, differentiation, migration and survival. Although there is variable expression of modulators of TGFβ action during differentiation, a differential effect on fat cell metabolism at the different stages of adipocyte differentiation was unclear. In the present study, 3T3L1 cells were used as an in vitro model to study the effect of TGFβ on adipogenic and thermogenic markers at various stages of preadipocyte to mature adipocyte differentiation. As in our earlier studies on the effect of TGFβ on CEBP’s, we used a standard differentiation mix, and one with the addition of rosiglitazone. RhTGFβ1 was added to undifferentiated adipocytes (preadipocytes) and to adipocytes at day 0 (commitment stage) as well as day 10 (terminal differentiation). Cellular responses in terms of Pref1, PPARγ, TLE3, PGC1α, PRDM16, UCP1 and UCP2 mRNA levels and selected protein products, were determined. Increases in PPARγ, PRDM16, UCP1 and UCP2 mRNA and decreases in Pref1 are good indicators of successful differentiation. The early addition of rhTGFβ1 during commitment stage decreased PPARγ, PRDM16, TLE3, UCP1 and UCP2 mRNA and decreased PRDM16 protein consistent with our earlier report on the inhibition of CEBP’s by TGFβ and CCN2. The addition of rhTGFβ1 to mature adipocyte at day 10 increased UCP1 mRNA and increased PRDM16 and UCP1 proteins. In the present study, our results suggest that TGFβ1 added late enhances the thermogenic potential of mature cells and causes 3T3L1 cells to differentiate to resemble brown or beige rather than white adipose tissue.
- Subjects :
- 0301 basic medicine
medicine.medical_treatment
Biophysics
030209 endocrinology & metabolism
White adipose tissue
Biology
Bioenergetics
adipocyte
Biochemistry
Molecular Bases of Health & Disease
Rosiglitazone
Transforming Growth Factor beta1
03 medical and health sciences
chemistry.chemical_compound
TGFβ
Mice
0302 clinical medicine
stages of differentiation
Adipocyte
3T3-L1 Cells
medicine
Adipocytes
Animals
RNA, Messenger
Molecular Biology
Research Articles
PRDM16
Adipogenesis
Cell growth
Thermogenesis
Cell Biology
Cell biology
030104 developmental biology
Cytokine
Metabolism
chemistry
Gene Expression Regulation
Transforming growth factor
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 15734935 and 01448463
- Volume :
- 40
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Bioscience Reports
- Accession number :
- edsair.doi.dedup.....e38d33529b94c55d3f945eb55c0860fe