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Characterization of a natural mouse monoclonal antibody recognizing epitopes shared by oxidized low-density lipoprotein and chaperonin 60 of Aggregatibacter actinomycetemcomitans
- Source :
- Immunologic research. 64(3)
- Publication Year :
- 2016
-
Abstract
- Natural antibodies are predominantly antibodies of the IgM isotype present in the circulation of all vertebrates that have not been previously exposed to exogenous antigens. They are often directed against highly conserved epitopes and bind to ligands of varying chemical composition with low affinity. In this study we cloned and characterized a natural mouse monoclonal IgM antibody selected by binding to malondialdehyde acetaldehyde epitopes on low-density lipoprotein (LDL). Interestingly, the IgM antibody cross-reacted with Aggregatibacter actinomycetemcomitans (Aa) bacteria, a key pathogenic microbe in periodontitis reported to be associated with risk factor for atherosclerosis, thus being named as Aa_Mab. It is more intriguing that the binding molecule of Aa to Aa_Mab IgM was found to be Aa chaperonin 60 or HSP60, a member of heat-shock protein family, behaving not only as a chaperone for correct protein folding but also as a powerful virulence factor of the bacteria for inducing bone resorption and as a putative pathogenic factor in atherosclerosis. The findings will highlight the question of whether molecular mimicry between pathogen components and oxidized LDL could lead to atheroprotective immune activity, and also would be of great importance in potential application of immune response-based preventive and therapeutic strategies against atherosclerosis and periodontal disease.
- Subjects :
- 0301 basic medicine
Risk
Protein Folding
medicine.drug_class
Immunology
Cross Reactions
medicine.disease_cause
Monoclonal antibody
Aggregatibacter actinomycetemcomitans
Epitope
Microbiology
03 medical and health sciences
Mice
0302 clinical medicine
Immune system
Antigen
medicine
Animals
Humans
Cloning, Molecular
Periodontitis
Antigens, Bacterial
biology
Molecular Mimicry
Antibodies, Monoclonal
030206 dentistry
Chaperonin 60
biology.organism_classification
Scavenger Receptors, Class E
Molecular mimicry
030104 developmental biology
Immunoglobulin M
biology.protein
Epitopes, B-Lymphocyte
HSP60
Antibody
Pasteurellaceae Infections
Subjects
Details
- ISSN :
- 15590755
- Volume :
- 64
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Immunologic research
- Accession number :
- edsair.doi.dedup.....e394116eae1c5f7dd34fdfc6b93f202c