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Zebrafish sip1a and sip1b are essential for normal axial and neural patterning
- Source :
- Developmental dynamics : an official publication of the American Association of Anatomists. 237(4)
- Publication Year :
- 2008
-
Abstract
- Smad-interacting protein-1 (SIP1) has been implicated in the development of Mowat-Wilson syndrome whose patients exhibit Hirschsprung disease, an aganglionosis of the large intestine, as well as other phenotypes. We have identified and cloned two sip1 orthologues in zebrafish. Both sip1 orthologues are expressed maternally and have dynamic zygotic expression patterns that are temporally and spatially distinct. We have investigated the function of both orthologues using translation and splice-blocking morpholino antisense oligonucleotides. Knockdown of the orthologues causes axial and neural patterning defects consistent with the previously described function of SIP1 as an inhibitor of BMP signaling. In addition, knockdown of both genes leads to a significant reduction/loss of the post-otic cranial neural crest. This results in a subsequent absence of neural crest precursors in the posterior pharyngeal arches and a loss of enteric precursors in the intestine.
- Subjects :
- animal structures
Body Patterning
Morpholino
Molecular Sequence Data
Biology
Article
Cranial neural crest
Morphogenesis
Animals
Humans
Protein Isoforms
Zebrafish
In Situ Hybridization
Gene knockdown
Base Sequence
Stem Cells
Intracellular Signaling Peptides and Proteins
Neural crest
Gene Expression Regulation, Developmental
Oligonucleotides, Antisense
Zebrafish Proteins
biology.organism_classification
Phenotype
Molecular biology
Neural Crest
embryonic structures
Enteric nervous system
Carrier Proteins
Developmental Biology
Subjects
Details
- ISSN :
- 10588388
- Volume :
- 237
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Developmental dynamics : an official publication of the American Association of Anatomists
- Accession number :
- edsair.doi.dedup.....e3946d0172e0ec4834d44809aaf89a3d