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In Vitro Genotoxicity Assessment from the Glycyrrhiza New Variety Extract

Authors :
Jong-Sik Jin
Jae-Ki Chang
Hyo-Jin An
Jeong Hoon Lee
Sa-Haeng Kang
Wonnam Kim
Ju-Ryoun Soh
Young-Jae Song
Yong-Deok Jeon
Jonghyun Lee
Dong-Gu Kim
Source :
Applied Sciences, Vol 11, Iss 10257, p 10257 (2021), Applied Sciences, Volume 11, Issue 21
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

The various species that comprise the genus Glycyrrhiza (Licorice) have long been used as oriental herbal medicines in Asian countries. Wongam (WG), which is a new variety of Glycyrrhiza, was developed in Korea to overcome the limitations of low productivity, environmental restrictions, and an insufficient presence of glycyrrhizic acid and liquiritigenin. In this study, we evaluated WG extract’s genotoxicity through an in vitro bacterial reverse mutation (AMES) test, an in vitro chromosome aberration test, and an in vivo mouse bone marrow micronucleus test. In the AMES test, WG extract at concentrations of up to 5000 µg/plate showed no genotoxicity regardless of S9 mix. No chromosome aberrations appeared after 6 h in 1400 µg/mL WG extract regardless of S9 mix or in 1100 µg/mL WG extract after 24 h without S9 mix. Nor was there a significant increase in the number of micronucleated polychromatic erythrocytes to total erythrocytes up to 5000 mg/kg/day for 2 days detected in the micronucleus test. These results confirm that WG extract is safe for use as an herbal medicine, as it precipitates no detectable genotoxic effects.

Details

Language :
English
ISSN :
20763417
Volume :
11
Issue :
10257
Database :
OpenAIRE
Journal :
Applied Sciences
Accession number :
edsair.doi.dedup.....e39fe072e5f147618307aa1c28898582