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In Vitro Genotoxicity Assessment from the Glycyrrhiza New Variety Extract
- Source :
- Applied Sciences, Vol 11, Iss 10257, p 10257 (2021), Applied Sciences, Volume 11, Issue 21
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- The various species that comprise the genus Glycyrrhiza (Licorice) have long been used as oriental herbal medicines in Asian countries. Wongam (WG), which is a new variety of Glycyrrhiza, was developed in Korea to overcome the limitations of low productivity, environmental restrictions, and an insufficient presence of glycyrrhizic acid and liquiritigenin. In this study, we evaluated WG extract’s genotoxicity through an in vitro bacterial reverse mutation (AMES) test, an in vitro chromosome aberration test, and an in vivo mouse bone marrow micronucleus test. In the AMES test, WG extract at concentrations of up to 5000 µg/plate showed no genotoxicity regardless of S9 mix. No chromosome aberrations appeared after 6 h in 1400 µg/mL WG extract regardless of S9 mix or in 1100 µg/mL WG extract after 24 h without S9 mix. Nor was there a significant increase in the number of micronucleated polychromatic erythrocytes to total erythrocytes up to 5000 mg/kg/day for 2 days detected in the micronucleus test. These results confirm that WG extract is safe for use as an herbal medicine, as it precipitates no detectable genotoxic effects.
- Subjects :
- Technology
QH301-705.5
QC1-999
medicine.disease_cause
Chromosome aberration
bacterial reverse mutation test
Ames test
chemistry.chemical_compound
In vivo
chromosome aberration test
medicine
General Materials Science
Glycyrrhiza
Biology (General)
Instrumentation
QD1-999
Fluid Flow and Transfer Processes
biology
Traditional medicine
Process Chemistry and Technology
Physics
genotoxicity
General Engineering
biology.organism_classification
Engineering (General). Civil engineering (General)
In vitro
Computer Science Applications
wongam
micronucleus test
Chemistry
chemistry
Micronucleus test
Liquiritigenin
TA1-2040
Genotoxicity
Subjects
Details
- Language :
- English
- ISSN :
- 20763417
- Volume :
- 11
- Issue :
- 10257
- Database :
- OpenAIRE
- Journal :
- Applied Sciences
- Accession number :
- edsair.doi.dedup.....e39fe072e5f147618307aa1c28898582