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ELECTROPHORETIC IDENTITY OF BIOLOGICALLY ACTIVE PITUITARY AND SERUM PROLACTINS IN THE LACTATING AND PERPHENAZINETREATED RAT
- Source :
- Acta Endocrinologica. 72:654-662
- Publication Year :
- 1973
- Publisher :
- Oxford University Press (OUP), 1973.
-
Abstract
- Polyacrylamide gel electrophoresis at neutral pH, 0°C, was used to test the identity of prolactin derived from serum and pituitary extracts of rats. Animals were studied after treatment with perphenazine, a prolactin releasing agent, and during post partum lactation. After gel electrophoresis, the position on the gel of prolactin activity was determined by the pigeon crop-sac bioassay. The biologically active prolactin exhibited identical electrophoretic mobility, whether derived from serum or pituitary, of either normal lactating or perphenazine treated rats.
- Subjects :
- medicine.medical_specialty
Chemistry
Endocrinology, Diabetes and Metabolism
Identity (social science)
Biological activity
General Medicine
Prolactin
Rats
Endocrinology
Biochemistry
Pregnancy
Pituitary Gland
Internal medicine
medicine
Animals
Lactation
Perphenazine
Pregnancy, Animal
Biological Assay
Electrophoresis, Polyacrylamide Gel
Female
Columbidae
Pituitary Hormone-Releasing Hormones
Subjects
Details
- ISSN :
- 1479683X and 08044643
- Volume :
- 72
- Database :
- OpenAIRE
- Journal :
- Acta Endocrinologica
- Accession number :
- edsair.doi.dedup.....e3c0015972d0dce6eafaf0e2e1c7b3ff