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Identification of MAL2, a novel member of the mal proteolipid family, though interactions with TPD52-like proteins in the yeast two-hybrid system
- Source :
- Genomics. 76(1-3)
- Publication Year :
- 2001
-
Abstract
- The TPD52 (tumor protein D52)-like proteins are small coiled-coil motif-bearing proteins which were first identified though their expression in human breast carcinoma. TPD52-like proteins are known to interact in hetero-and homomeric fashions, but there are no known heterologous binding partners for these proteins. We now report the cloning of a novel member of the MAL proteolipid family, named MAL2, though its interaction with a TPD52L2 bait in a yeast two-hybrid screen. MAL2 is predicted to be 176 residues (19 kDa) with four transmembrane domains and is 35.8% identical to MAL, a proteolipid required in apical vesicle transport. The MAL2 prey bound all TPD52-like baits tested in the yeast two-hybrid system and in vitro translation of MAL2 produced a single 19-kDa (35)S-labeled protein which specifically bound full-length GST-Tpd52 in GST pull-down assays. The gene MAL2, which was localized to human chromosomal band 8q23 and shown to consist of four exons, is predominantly expressed in human kidney, lung, and liver. Our study has therefore identified a novel member of the MAL proteolipid family and potentially implicates TPD52-like proteins in vesicle transport.
- Subjects :
- DNA, Complementary
Two-hybrid screening
Proteolipids
Molecular Sequence Data
Vesicular Transport Proteins
Breast Neoplasms
Plasma protein binding
Two-Hybrid System Techniques
Genetics
Tumor Cells, Cultured
Humans
Amino Acid Sequence
RNA, Messenger
Peptide sequence
Glutathione Transferase
biology
Membrane transport protein
Myelin and Lymphocyte-Associated Proteolipid Proteins
Chromosome Mapping
Membrane Transport Proteins
Molecular biology
Recombinant Proteins
Transport protein
Cell biology
Neoplasm Proteins
Transmembrane domain
Protein Transport
Tumor Protein D52
biology.protein
Carrier Proteins
Myelin Proteins
Chromosomes, Human, Pair 8
Protein Binding
Subjects
Details
- ISSN :
- 08887543
- Volume :
- 76
- Issue :
- 1-3
- Database :
- OpenAIRE
- Journal :
- Genomics
- Accession number :
- edsair.doi.dedup.....e3c813d85c1a5de60646c1131fea71eb