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Secondary metabolic profiles and anticancer actions from fruit extracts of immature pomegranates
- Source :
- PLoS ONE, PLoS ONE, Vol 16, Iss 8 (2021), PloS one 16 (2021). doi:10.1371/journal.pone.0255831, info:cnr-pdr/source/autori:Russo, Venera; Continella, Alberto; Drago, Carmelo; Gentile, Alessandra; Malfa, Stefano La; Leotta, Claudia Giovanna; Pulvirenti, Luana; Ruberto, Giuseppe; Pitari, Giovanni Mario; Siracusa, Laura/titolo:Secondary metabolic profiles and anticancer actions from fruit extracts of immature pomegranates/doi:10.1371%2Fjournal.pone.0255831/rivista:PloS one/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume:16, PLoS ONE, Vol 16, Iss 8, p e0255831 (2021)
- Publication Year :
- 2021
-
Abstract
- Immature fruits from Punica granatum L. thinning are a neglected side product of pomegranate production with cumbersome disposal costs for farmers. To explore value potential of immature fruits from pomegranate ‘Wonderful’ cultivars, the compositional landscapes and antitumorigenic activities of pomegranate extracts from two different stages of maturation were assessed. Cancer cell proliferation and cytotoxicity was quantified in human lung H1299 and colon HCT116 adenocarcinomas by crystal violet staining, MTS assay and caspase-3 activity. High performance liquid chromatography—diode array detector (HPLC/DAD) and high performance liquid chromatography—electrospray ionization—mass spectrometry (HPLC/ESI-MS) analyses indicate that immature fruits are rich sources of gallotannins and ellagitannins, with the highest amounts contained in immature fruit peels. Biological investigations reveal a robust anticancer activity by those immature P. granatum fruit extracts, which reflected induction of tumor cytotoxicity and cell death mechanisms. Together, present observations suggest P. granatum byproducts from the thinning process may provide unexplored values for virtuous circular economy.
- Subjects :
- Cancer Treatment
Apoptosis
Biochemistry
Pomegranate
Lung and Intrathoracic Tumors
Isomers
Stereochemistry
Medicine and Health Sciences
Metabolites
Cultivar
Food science
Cytotoxicity
Chromatography, High Pressure Liquid
Multidisciplinary
biology
Adenocarcinoma of the Lung
Cell Death
food and beverages
Eukaryota
Plants
Hydrolyzable Tannins
Chemistry
anticancer activity
Oncology
Cell Processes
Punica
Physical Sciences
Tumor cytotoxicity
Medicine
Research Article
Programmed cell death
Spectrometry, Mass, Electrospray Ionization
Fruit extracts
Science
Adenocarcinoma
Fruits
Isomerism
ellagitannins
Cell Line, Tumor
Humans
Cell Proliferation
Colorectal Cancer
Plant Extracts
Carcinoma
Chemical Compounds
Organisms
Cancers and Neoplasms
Biology and Life Sciences
Cell Biology
biology.organism_classification
Antineoplastic Agents, Phytogenic
Staining
Metabolism
Fruit
fruit thinning
gallotannins
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, Vol 16, Iss 8 (2021), PloS one 16 (2021). doi:10.1371/journal.pone.0255831, info:cnr-pdr/source/autori:Russo, Venera; Continella, Alberto; Drago, Carmelo; Gentile, Alessandra; Malfa, Stefano La; Leotta, Claudia Giovanna; Pulvirenti, Luana; Ruberto, Giuseppe; Pitari, Giovanni Mario; Siracusa, Laura/titolo:Secondary metabolic profiles and anticancer actions from fruit extracts of immature pomegranates/doi:10.1371%2Fjournal.pone.0255831/rivista:PloS one/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume:16, PLoS ONE, Vol 16, Iss 8, p e0255831 (2021)
- Accession number :
- edsair.doi.dedup.....e3d0f658fab2aa25fba283f3ea28552e