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shRNA transgenic swine display resistance to infection with the foot-and-mouth disease virus
- Source :
- Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021), Scientific Reports
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Foot-and-mouth disease virus (FMDV) is one of the most important animal pathogens in the world. FMDV naturally infects swine, cattle, and other cloven-hoofed animals. FMD is not adequately controlled by vaccination. An alternative strategy is to develop swine that are genetically resistant to infection. Here, we generated FMDV-specific shRNA transgenic cells targeting either nonstructural protein 2B or polymerase 3D of FMDV. The shRNA-positive transgenic cells displayed significantly lower viral production than that of the control cells after infection with FMDV (P +, CD4+, CD8+, CD21+, and CD172+ cells, and the production of IFN-γ were analyzed, there were no significant differences observed between the TGCS and NS or Non-TGCS, suggesting that the FMDV resistance may be mainly derived from the RNAi-based antiviral pathway. Our work provides a foundation for a breeding approach to preventing infectious disease in swine.
- Subjects :
- Swine
viruses
Science
animal diseases
Viremia
Article
Virus
Animals, Genetically Modified
Small hairpin RNA
Immune system
RNA interference
medicine
Animals
RNA, Small Interfering
Disease Resistance
Swine Diseases
Multidisciplinary
biology
medicine.disease
biology.organism_classification
Virology
Foot-and-Mouth Disease Virus
Foot-and-Mouth Disease
Genetic engineering
biology.protein
RNA, Viral
Medicine
RNA Interference
Foot-and-mouth disease virus
Antibody
CD8
Cloning
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....e3e9af72d29bcfb10cf6ff3b21c20021