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The expression of the sarco/endoplasmic reticulum Ca2+-ATPases in thyroid and its down-regulation following neoplastic transformation
The expression of the sarco/endoplasmic reticulum Ca2+-ATPases in thyroid and its down-regulation following neoplastic transformation
- Source :
- Journal of molecular endocrinology 30 (2003): 399–409., info:cnr-pdr/source/autori:Pacifico F., Ulianich L., De Micheli S., Treglia S., Leonardi A., Vito P., Formisano S., Consiglio E., Di Jeso B./titolo:The expression of the sarco%2Fendoplasmic reticulum Ca2+-ATPases in thyroid and its down-regulation following neoplastic transformation./doi:/rivista:Journal of molecular endocrinology/anno:2003/pagina_da:399/pagina_a:409/intervallo_pagine:399–409/volume:30
- Publication Year :
- 2003
- Publisher :
- Pubblicata da: SOC ENDOCRINOLOGY, 22 APEX COURT, WOODLANDS, BRADLEY STOKE, BRISTOL, ENGLAND, BS32 4JT primo editore: Journal of Endocrinology Limited:17 18 Courtyard Woodlands, Bristol BS12 4NQ United Kingdom:011 44 117 9616046, EMAIL: sxa15@psu.edu, INTERNET: http://www.psu.edu, Fax: 011 44 117 9616071, 2003.
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Abstract
- Maintaining a high Ca(2+) concentration in the lumen of the endoplasmic reticulum (ER), by the action of sarco/endoplasmic reticulum Ca(2+)-ATPases (SERCAs), is important in many cellular processes, such as Ca(2+)-mediated cytosolic signaling in response to extracellular stimuli, cell growth and proliferation, and synthesis, processing and folding of ER-translated proteins. In the thyroid gland, SERCAs have not been studied yet, and there is little information available on general problems such as the expression of SERCAs following neoplastic transformation. In this study we investigated the expression of SERCA2b and SERCA3 in rat thyroid tIssue and, in addition, in normal and transformed rat thyroid cell lines. RT-PCR and Northern blot assays showed that SERCA2b is the SERCA form preferentially expressed in the thyroid. In rat thyroid, SERCA2b mRNA was expressed at a higher level than that of other non-muscle tIssues such as liver or spleen, but at much lower level than in brain. On the other hand, SERCA3 mRNA was not detected in thyroid by Northern blot analysis, or barely detected by RT-PCR assays. We also studied the SERCA2b expression pattern in PC Cl3 thyroid cells transformed by several oncogenes that induce different degrees of malignancy and dedifferentiation. RT-PCR and Northern blot assays showed that SERCA2b mRNA expression dramatically decreased in highly tumorigenic thyroid cells, while expression of glyceraldehyde-3-phosphate dehydrogenase mRNA, a housekeeping gene used as internal control, exhibited no variations. The dramatic down-regulation of SERCA2b expression in fully transformed thyroid cells was also evident by Western blot analysis. Also, following neoplastic transformation of thyroid cells, the enzymatic activity of SERCA2b was reduced in a measure which correlated with the mRNA and protein levels. Therefore, rat thyrocytes expressed intermediate levels of SERCAs, mostly the SERCA2b isoform. This pattern of expression was basically reproduced in fully differentiated thyroid cells in culture and was sensitive to neoplastic transformation.
- Subjects :
- SERCA
Blotting, Western
Thyroid Gland
Down-Regulation
Calcium-Transporting ATPases
Biology
Cell Line
Sarcoplasmic Reticulum Calcium-Transporting ATPases
Endocrinology
Western blot
medicine
Animals
Neoplastic transformation
Northern blot
Molecular Biology
medicine.diagnostic_test
Reverse Transcriptase Polymerase Chain Reaction
Endoplasmic reticulum
Thyroid
Blotting, Northern
Molecular biology
Rats
Blot
Cell Transformation, Neoplastic
medicine.anatomical_structure
Cell culture
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Journal of molecular endocrinology 30 (2003): 399–409., info:cnr-pdr/source/autori:Pacifico F., Ulianich L., De Micheli S., Treglia S., Leonardi A., Vito P., Formisano S., Consiglio E., Di Jeso B./titolo:The expression of the sarco%2Fendoplasmic reticulum Ca2+-ATPases in thyroid and its down-regulation following neoplastic transformation./doi:/rivista:Journal of molecular endocrinology/anno:2003/pagina_da:399/pagina_a:409/intervallo_pagine:399–409/volume:30
- Accession number :
- edsair.doi.dedup.....e43805b58dbc5ab2036161094a9bc1c5