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Gut Microbial Protein Expression in Response to Dietary Patterns in a Controlled Feeding Study: A Metaproteomic Approach
- Source :
- Microorganisms, Microorganisms, Vol 8, Iss 3, p 379 (2020), Volume 8, Issue 3
- Publication Year :
- 2020
- Publisher :
- MDPI, 2020.
-
Abstract
- Although the gut microbiome has been associated with dietary patterns linked to health, microbial metabolism is not well characterized. This ancillary study was a proof of principle analysis for a novel application of metaproteomics to study microbial protein expression in a controlled dietary intervention. We measured the response of the microbiome to diet in a randomized crossover dietary intervention of a whole-grain, low glycemic load diet (WG) and a refined-grain, high glycemic load diet (RG). Total proteins in stools from 9 participants at the end of each diet period (n = 18) were analyzed by LC MS/MS and proteins were identified using the Human Microbiome Project (HMP) human gut microbiome database and UniProt human protein databases. T-tests, controlling for false discovery rate (FDR) &lt<br />10%, were used to compare the Gene Ontology (GO) biological processes and bacterial enzymes between the two interventions. Using shotgun proteomics, more than 53,000 unique peptides were identified including microbial (89%) and human peptides (11%). Forty-eight bacterial enzymes were statistically different between the diets, including those implicated in SCFA production and degradation of fatty acids. Enzymes associated with degradation of human mucin were significantly enriched in the RG diet. These results illustrate that the metaproteomic approach is a valuable tool to study the microbial metabolism of diets that may influence host health.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
030106 microbiology
Biology
Proteomics
Microbiology
Article
03 medical and health sciences
chemistry.chemical_compound
proteomics
dietary intervention
Virology
Glycemic load
medicine
Food science
Microbiome
Shotgun proteomics
lcsh:QH301-705.5
Fatty acid metabolism
dysbiosis
medicine.disease
SCFA
030104 developmental biology
lcsh:Biology (General)
chemistry
fatty acid metabolism
mucin-degrading enzymes
Metaproteomics
Dysbiosis
Human Microbiome Project
Subjects
Details
- Language :
- English
- ISSN :
- 20762607
- Volume :
- 8
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Microorganisms
- Accession number :
- edsair.doi.dedup.....e43a8704f31ec158d3d2d332e1ae1f74