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Organ-specific metabolic profiles of the liver and kidney during brain death and afterwards during normothermic machine perfusion of the kidney
- Source :
- American Journal of Transplantation, 20(9), 2425-2436. Wiley, van Erp, A C, Qi, H, Jespersen, N R, Hjortbak, M V, Ottens, P J, Wiersema-Buist, J, Nørregaard, R, Pedersen, M, Laustsen, C, Leuvenink, H G D & Jespersen, B 2020, ' Organ-specific metabolic profiles of the liver and kidney during brain death and afterwards during normothermic machine perfusion of the kidney ', American Journal of Transplantation, vol. 20, no. 9, pp. 2425-2436 . https://doi.org/10.1111/ajt.15885, American Journal of Transplantation
- Publication Year :
- 2020
-
Abstract
- We investigated metabolic changes during brain death (BD) using hyperpolarized magnetic resonance (MR) spectroscopy and ex vivo graft glucose metabolism during normothermic isolated perfused kidney (IPK) machine perfusion. BD was induced in mechanically ventilated rats by inflation of an epidurally placed catheter; sham‐operated rats served as controls. Hyperpolarized [1‐13C]pyruvate MR spectroscopy was performed to quantify pyruvate metabolism in the liver and kidneys at 3 time points during BD, preceded by injecting hyperpolarized[1‐13C]pyruvate. Following BD, glucose oxidation was measured using tritium‐labeled glucose (d‐6‐3H‐glucose) during IPK reperfusion. Quantitative polymerase chain reaction and biochemistry were performed on tissue/plasma. Immediately following BD induction, lactate increased in both organs (liver: eµd0.21, 95% confidence interval [CI] [−0.27, −0.15]; kidney: eµd0.26, 95% CI [−0.40, −0.12]. After 4 hours of BD, alanine production decreased in the kidney (eµd0.14, 95% CI [0.03, 0.25], P<br />Differences in glucose metabolism in the liver and kidney can be visualized in vivo during brain death using hyperpolarized magnetic resonance spectroscopy and ex vivo following brain death during normothermic kidney machine perfusion using glucose oxidation.
- Subjects :
- Nephrology
In vivo magnetic resonance spectroscopy
basic (laboratory) research/science
translational research/science
donors and donation
graft survival
basic (laboratory) research
kidney transplantation/nephrology
030230 surgery
Kidney
GLUCOSE
0302 clinical medicine
Basic Science
MAGNETIC-RESONANCE
Immunology and Allergy
Pharmacology (medical)
donation after brain death (DBD) [donors and donation]
donors and donation: donation after brain death (DBD)
science
dysfunction
liver transplantation
Organ Preservation
animal models
Perfusion
medicine.anatomical_structure
Liver
Metabolome
Original Article
Brain Death
medicine.medical_specialty
liver allograft function/dysfunction
liver allograft function
kidney (allograft) function
nephrology
kidney transplantation
organ procurement and allocation
kidney (allograft) function/dysfunction
DONOR
03 medical and health sciences
Internal medicine
medicine
Animals
RENAL VIABILITY
donation after brain death (DBD)
Machine perfusion
business.industry
TRANSPLANTATION
REDOX STATE
GRAFT
HYPERPOLARIZED C-13
GLUCONEOGENESIS
Rats
Transplantation
Endocrinology
Gluconeogenesis
translational research
hepatology
ORIGINAL ARTICLES
business
liver transplantation/hepatology
Ex vivo
KETONE-BODY RATIO
Subjects
Details
- Language :
- English
- ISSN :
- 16006135
- Database :
- OpenAIRE
- Journal :
- American Journal of Transplantation, 20(9), 2425-2436. Wiley, van Erp, A C, Qi, H, Jespersen, N R, Hjortbak, M V, Ottens, P J, Wiersema-Buist, J, Nørregaard, R, Pedersen, M, Laustsen, C, Leuvenink, H G D & Jespersen, B 2020, ' Organ-specific metabolic profiles of the liver and kidney during brain death and afterwards during normothermic machine perfusion of the kidney ', American Journal of Transplantation, vol. 20, no. 9, pp. 2425-2436 . https://doi.org/10.1111/ajt.15885, American Journal of Transplantation
- Accession number :
- edsair.doi.dedup.....e4abebbb1c8300c4b0e4a5afa8b47010