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Resveratrol pretreatment attenuates injury and promotes proliferation of neural stem cells following oxygen-glucose deprivation/reoxygenation by upregulating the expression of Nrf2, HO-1 and NQO1 in vitro
- Source :
- Molecular Medicine Reports
- Publication Year :
- 2016
- Publisher :
- D.A. Spandidos, 2016.
-
Abstract
- There is considerable interest in the use of drugs and other methods for protecting implanted neural stem cells (NSCs) from the adverse environment of injured tissue for successful cell therapy. Resveratrol can modify cardiac stem cells to enhance their survival and differentiation, however, its effect and the mechanism underlying its neuroprotective effect on NSCs following stroke remain to be fully elucidated. Nuclear factor erythroid 2-related factor 2 (Nrf-2) signaling is important in antioxidative stress, and the role of Nrf-2 signaling in the enhanced neuroprotection of NSCs by resveratrol following stroke also remains to be elucidated. In the present study, NSCs were pretreated with resveratrol prior to oxygen-glucose deprivation/reoxygenation (OGD/R) in vitro. The survival, apoptosis and proliferation of the NSCs were assessed using an MTT assay, Hoechst 33258 staining of nuclei and flow cytometry, respectively. In addition, the activity of superoxide dismutase (SOD), level of malondiadehyde (MDA) and content of glutathione (GSH) were determined. The protein expressions levels of Nrf-2, NAD(P)H:quinone oxidoreductase 1 (NQO-1), and heme oxygenase 1 (HO-1) were detected using western blot analysis. It was found that resveratrol markedly enhanced NSC survival and proliferation, decreased apoptosis and the levels of MDA, and increased the activity of SOD and content of GSH in a concentration-dependent manner following OGD/R injury in vitro. In addition, the protein expression levels of Nrf2, HO-1 and NQO1 were significantly upregulated. These findings suggested that resveratrol attenuated injury and promoted proliferation of the NSCs, at least in part, by upregulating the expression of Nrf2, HO-1 and NQO1 following OGD/R injury in vitro.
- Subjects :
- 0301 basic medicine
Male
Cancer Research
NF-E2-Related Factor 2
Pharmacology
Resveratrol
resveratrol
Biochemistry
Neuroprotection
Antioxidants
Superoxide dismutase
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Neural Stem Cells
Stilbenes
Genetics
NAD(P)H Dehydrogenase (Quinone)
Animals
MTT assay
Molecular Biology
Cells, Cultured
Cell Proliferation
biology
apoptosis
Articles
Molecular biology
Neural stem cell
Heme oxygenase
Oxygen
Oxidative Stress
030104 developmental biology
Glucose
Neuroprotective Agents
Oncology
chemistry
nervous system
Apoptosis
biology.protein
Molecular Medicine
Female
Stem cell
030217 neurology & neurosurgery
oxygen-glucose deprivation/reoxygenation
Heme Oxygenase-1
Subjects
Details
- Language :
- English
- ISSN :
- 17913004 and 17912997
- Volume :
- 14
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....e4d586afa3df6a683ceb79206a387c0d