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MIIP gene expression is associated with radiosensitivity in human nasopharyngeal carcinoma cells
- Source :
- Oncology Letters.
- Publication Year :
- 2018
- Publisher :
- Spandidos Publications, 2018.
-
Abstract
- The present study aims to investigate the radiosensitization effect of the migration and invasion inhibitory protein (MIIP) gene on nasopharyngeal carcinoma (NPC) cells. The MIIP gene was transfected into NPC 5-8F and CNE2 cells. The level of MIIP was analyzed by quantitative reverse transcription-polymerase chain reaction analysis and western blot. The changes in radiosensitivity of the cells were analyzed by colony formation assay. The changes in cell apoptosis and cycle distribution following irradiation were detected by flow cytometry. The expression of BCL2 associated X, apoptosis regulator/B-cell lymphoma 2 was evaluated using western blot. DNA damage was analyzed by counting γ-H2AX foci. The expression levels of γ-H2AX were evaluated by immunofluorescence and western blot. In a previous study by the authors, the results indicated that the expression of MIIP gene evidently increased in MIIP-transfected 5-8F (5–8F OE) and MIIP-transfected CNE2 (CNE2 OE) cells compared with the parental or negative control cells. In the present study, the survival rate of 5-8F OE and CNE2 OE cells markedly decreased following irradiation (0, 2, 4, 6 and 8 Gy) compared with the negative control (5–8F NC and CNE2 NC) and the untreated (5–8F and CNE2) groups. The expression of MIIP was able to increase apoptosis, which resulted in G2/M cell cycle arrest and DNA damage repair was attenuated in 5-8F and CNE2 cells following irradiation as measured by the accumulation of γ-H2AX. It was indicated that MIIP expression is associated with the radiosensitivity of NPC cells and has a significant role in regulating cell radiosensitivity.
- Subjects :
- 0301 basic medicine
Cancer Research
medicine.diagnostic_test
Chemistry
Articles
Transfection
Cell cycle
medicine.disease
Molecular biology
Flow cytometry
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Oncology
Nasopharyngeal carcinoma
Western blot
Apoptosis
030220 oncology & carcinogenesis
Gene expression
medicine
Radiosensitivity
Subjects
Details
- ISSN :
- 17921082 and 17921074
- Database :
- OpenAIRE
- Journal :
- Oncology Letters
- Accession number :
- edsair.doi.dedup.....e4f4695e445f6d119c15108148d7cbac