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Diversity of phlebotomine sand flies and molecular detection of trypanosomatids in Brumadinho, Minas Gerais, Brazil
- Source :
- PLoS ONE, Vol 15, Iss 6, p e0234445 (2020), PLoS ONE
- Publication Year :
- 2020
- Publisher :
- Public Library of Science (PLoS), 2020.
-
Abstract
- This study aimed to describe the sand fly fauna and detect trypanosomatids in these insects from Casa Branca, state of Minas Gerais, Brazil, an endemic area of both visceral (VL) and tegumentary leishmaniasis (TL). Sand flies were collected bimonthly from May 2013 to July 2014, using automatic light traps exposed for three consecutive nights in peridomiciliary areas of nine houses with previous reports of VL and TL. ITS1-PCR and DNA sequencing were performed for trypanosomatids identification. A total of 16,771 sand flies were collected belonging to 23 species. The most abundant species was Nyssomyia whitmani (Antunes & Coutinho, 1939) (70.9%), followed by Lutzomyia longipalpis (Lutz & Neiva, 1912) (15.2%) and Migonemyia migonei (França, 1920) (9.1%). Leishmania amazonensis DNA was detected in Ny. whitmani (four pools) and Le. braziliensis DNA was detected in Psychodopygus lloydi (one pool). In seven pools of Ny. whitmani and in one pool of Lu. longipalpis positive for Leishmania DNA, the parasite species was not determined due to the low quality of the sequences. Moreover, DNA of Herpetomonas spp. was detected in Ny. whitmani (two pools) and Cortelezzii complex (one pool). DNA of Crithidia spp. was detected in Ny. whitmani and Ps. lloydi (both one pool). Our results suggest that Ny. whitmani may be involved in the transmission of Le. amazonensis in the study area. The molecular detection of Le. amazonensis suggests the presence of this species in a sylvatic cycle between vertebrate and invertebrate hosts in the region of Casa Branca. Our data also reveal the occurrence of other non-Leishmania trypanosomatids in sand flies in Casa Branca District.
- Subjects :
- 0301 basic medicine
Endemic Diseases
Tegumentary leishmaniasis
Fauna
Lutzomyia longipalpis
Disease Vectors
Geographical locations
0302 clinical medicine
Zoonoses
Crithidia
Medicine and Health Sciences
Parasite hosting
Leishmaniasis
Protozoans
Leishmania
Multidisciplinary
Ecology
biology
Eukaryota
Biodiversity
Infectious Diseases
Medicine
Female
Sylvatic cycle
Brazil
Research Article
Neglected Tropical Diseases
Trypanosoma
Ecological Metrics
Science
030231 tropical medicine
Zoology
DNA sequencing
03 medical and health sciences
parasitic diseases
Parasitic Diseases
Animals
Humans
Protozoan Infections
Ecology and Environmental Sciences
fungi
Organisms
Biology and Life Sciences
Species Diversity
Sequence Analysis, DNA
South America
DNA, Protozoan
Tropical Diseases
biology.organism_classification
Parasitic Protozoans
Insect Vectors
Sand Flies
Vector-Borne Diseases
Species Interactions
030104 developmental biology
Phlebotomus
People and places
Psychodidae
Leishmania DNA
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 15
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....e5176cf9550d7d7f43a6a8d129f43f4a