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Transcriptomic and Proteomic Research To Explore Bruchid-Resistant Genes in Mungbean Isogenic Lines
- Source :
- Journal of agricultural and food chemistry. 64(34)
- Publication Year :
- 2016
-
Abstract
- Mungbean (Vigna radiata (L.) Wilczek) is an important rotation legume crop for human nutrition in Asia. Bruchids (Callosobruchus spp.) currently cause heavy damage as pests of grain legumes during storage. We used omics-related technologies to study the mechanisms of bruchid resistance in seeds of the nearly isogenic lines VC1973A (bruchid-susceptible) and VC6089A (bruchid-resistant). A total of 399 differentially expressed genes (DEGs) were identified between the two lines by transcriptome sequencing. Among these DEGs, 251 exhibited high expression levels and 148 expressed low expression levels in seeds of VC6089A. Forty-five differential proteins (DPs) were identified by isobaric tags for relative and absolute quantification (iTRAQ); 21 DPs had higher abundances in VC6089A, and 24 DPs had higher abundances in VC1973A. According to transcriptome and proteome data, only three DEGs/DPs, including resistant-specific protein (g39185), gag/pol polyprotein (g34458), and aspartic proteinase (g5551), were identified and located on chromosomes 5, 1, and 7, respectively. Both g39185 and g34458 genes encode a protein containing a BURP domain. In previous research on bruchid molecular markers, the g39185 gene located close to the molecular markers of major bruchid-resistant locus may be a bruchid-resistant gene.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Proteomics
food.ingredient
Locus (genetics)
Biology
01 natural sciences
Transcriptome
Vigna
03 medical and health sciences
food
Botany
Animals
Gene
Legume
Disease Resistance
Plant Diseases
Plant Proteins
Genetics
General Chemistry
biology.organism_classification
Coleoptera
030104 developmental biology
Callosobruchus
Proteome
General Agricultural and Biological Sciences
BURP domain
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15205118
- Volume :
- 64
- Issue :
- 34
- Database :
- OpenAIRE
- Journal :
- Journal of agricultural and food chemistry
- Accession number :
- edsair.doi.dedup.....e52f14174df5ea1e3b673b41f68369c2