Back to Search
Start Over
Disparate E-cadherin mutations in LCIS and associated invasive breast carcinomas
- Source :
- Molecular Pathology. 54:91-97
- Publication Year :
- 2001
- Publisher :
- BMJ, 2001.
-
Abstract
- Aims—The relation between lobular carcinoma in situ (LCIS) and invasive breast cancer is unresolved. In an attempt to establish whether LCIS is a precursor of invasive cancer the mutational status and the expression of E-cadherin was analysed in LCIS and associated invasive breast carcinoma in 23 patients. Methods—Foci of LCIS and associated invasive carcinoma were individually microdissected from tissue from 23 patients. Exons 4–16 of the E-cadherin gene were analysed using single strand conformation polymorphism (SSCP); protein expression and the localisation of E-cadherin and β-catenin were assessed with the use of immunohistochemistry. Results—Immunohistochemistry revealed a lack of expression of E-cadherin and β-catenin in most LCIS samples and invasive foci. In all but four cases, the staining pattern was identical in the LCIS and associated invasive areas. When E-cadherin was absent, β-catenin was also undetected, suggesting a lack of expression of alternative classic cadherin members in these lesions. Coincident E-cadherin mutations in LCIS and associated invasive carcinoma were not identified in this series of patients. However, mutational analysis of E-cadherin in multiple foci of carcinoma in situ surrounding an invasive lesion provided evidence to support ductal carcinoma in situ as a precursor of invasive ductal carcinoma. Conclusion—These data support the hypothesis that LCIS is not a precursor of invasive breast carcinoma but a marker of increased risk of developing invasive disease.
- Subjects :
- Paper
Pathology
medicine.medical_specialty
Lobular carcinoma
Mammary gland
Breast Neoplasms
Biology
Pathology and Forensic Medicine
Breast cancer
medicine
Humans
Neoplasm Invasiveness
skin and connective tissue diseases
Polymorphism, Single-Stranded Conformational
beta Catenin
Microdissection
Carcinoma in situ
Ductal carcinoma
Cadherins
medicine.disease
Neoplasm Proteins
Carcinoma, Lobular
Cytoskeletal Proteins
medicine.anatomical_structure
Tumor progression
Mutation
Disease Progression
Trans-Activators
Immunohistochemistry
Female
Carcinoma in Situ
Subjects
Details
- ISSN :
- 13668714
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Molecular Pathology
- Accession number :
- edsair.doi.dedup.....e55cd1fb907ed70d1ffdc8a33198a9b3