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High-resolution respirometry for evaluation of mitochondrial function on brain and heart homogenates in a rat model of cardiac arrest and cardiopulmonary resuscitation
- Source :
- Biomedicine & Pharmacotherapy, Vol 142, Iss, Pp 111935-(2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- The physiology and physiopathology process of mitochondrial function following cardiac arrest remains poorly understood. We aimed to assess mitochondrial respiratory function on the heart and brain homogenates from cardiac arrest rats. The expression level of SIRT1/PGC-1α pathway was measured by immunoblotting. 30 rats were assigned to the CA group and the sham group. Rats of CA were subjected to 6 min of untreated ventricular fibrillation (VF) followed by 8 min of cardiopulmonary resuscitation (CPR). Mitochondrial respiratory function was compromised following CA and I/R injury, as indicated by CIL (451.46 ± 71.48 vs. 909.91 ± 5.51 pmol/min*mg for the heart and 464.14 ± 8.22 vs. 570.53 ± 56.33 pmol/min*mg for the brain), CI (564.04 ± 64.34 vs. 2729.52 ± 347.39 pmol/min*mg for the heart and 726.07 ± 85.78 vs. 1762.82 ± 262.04 pmol/min*mg for the brain), RCR (1.88 ± 0.46 vs. 3.57 ± 0.38 for the heart and 2.05 ± 0.19 vs. 3.49 ± 0.19, for the brain) and OXPHOS coupling efficiency (0.45 ± 0.11 vs. 0.72 ± 0.03 for the heart and 0.52 ± 0.05 vs. 0.71 ± 0.01 for the brain). However, routine respiration was lower in the heart and comparable in the brain after CA. CIV did not change in the heart but was enhanced in the brain. Furthermore, both SIRT1 and PGC-1α were downregulated concurrently in the heart and brain. The mitochondrial respiratory function was compromised following CA and I/R injury, and the major affected respiratory state is complex I-linked respiration. Furthermore, the heart and the brain respond differently to the global I/R injury after CA in mitochondrial respiratory function. Inhibition of the SIRT1/PGC-1α pathway may be a major contributor to the impaired mitochondrial respiratory function.
- Subjects :
- Male
medicine.medical_specialty
medicine.medical_treatment
Ischemia-reperfusion injury
RM1-950
Oxidative phosphorylation
High-resolution respirometry
Mitochondrial respiratory function
Rats, Sprague-Dawley
Sirtuin 1
Internal medicine
Respiration
medicine
Animals
Respiratory function
Cardiopulmonary resuscitation
Respiratory system
Biological Oxygen Demand Analysis
Pharmacology
business.industry
Myocardium
Brain
General Medicine
Cardiac arrest
medicine.disease
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
Cardiopulmonary Resuscitation
Pathophysiology
Heart Arrest
Mitochondria
Disease Models, Animal
Endocrinology
Spirometry
Reperfusion Injury
Ventricular Fibrillation
Ventricular fibrillation
SIRT1/PGC-1α pathway
Therapeutics. Pharmacology
business
Function (biology)
Signal Transduction
Subjects
Details
- ISSN :
- 07533322
- Volume :
- 142
- Database :
- OpenAIRE
- Journal :
- Biomedicine & Pharmacotherapy
- Accession number :
- edsair.doi.dedup.....e57519e6529b195825be1775d8e2bbb7