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Immunoephelometric determination of fibrinogen and its derivatives in plasma
- Source :
- Journal of immunological methods. 1(3)
- Publication Year :
- 1972
-
Abstract
- An immunonephelometric method for determination of fibrinogen and fibrin degradation products in plasma is described. Test plasms are diluted 1/600 in a solution containing 17 g NaCl and 3.8 g trisodium citrate/100 ml. To 3 cm 3 of this mixture 0.2 cm 3 of a concentrated antifibrin gamma globulin preparation is added. The reading of this sample minus the γ-globulin blank in a Thorp Nephelometer is directly proportional to the fibrinogen content of the plasma or to its FDP concentration in terms of intact fibrinogen. This paper examines the mechanism, accuracy and reproducibility of this method.
- Subjects :
- Immunodiffusion
Nitrogen
Immunology
Antigen-Antibody Complex
Fibrinogen
Fibrin
Antibodies
chemistry.chemical_compound
Iodine Isotopes
medicine
Methods
Immunology and Allergy
Animals
Humans
Streptokinase
Fibrinolysin
Antigens
Trisodium citrate
Reproducibility
Chromatography
Sheep
biology
Immune Sera
Immunochemistry
Gamma globulin
Plasma
chemistry
Solubility
biology.protein
Blood Coagulation Tests
gamma-Globulins
medicine.drug
Densitometry
Subjects
Details
- ISSN :
- 00221759
- Volume :
- 1
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of immunological methods
- Accession number :
- edsair.doi.dedup.....e58dd0bd2309df165341dcca72dc2923