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Hydrogen Sulfide Mediated Inhibitory Neurotransmission to the Pig Bladder Neck: Role of K ATP Channels, Sensory Nerves and Calcium Signaling
- Source :
- Journal of Urology. 190:746-756
- Publication Year :
- 2013
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2013.
-
Abstract
- Because neuronal released endogenous H2S has a key role in relaxation of the bladder outflow region, we investigated the mechanisms involved in H2S dependent inhibitory neurotransmission to the pig bladder neck.Bladder neck strips were mounted in myographs for isometric force recording and simultaneous measurement of intracellular Ca(2+) and tension.On phenylephrine contracted preparations electrical field stimulation and the H2S donor GYY4137 evoked frequency and concentration dependent relaxation, which was reduced by desensitizing capsaicin sensitive primary afferents with capsaicin, and the blockade of adenosine 5'-triphosphate dependent K(+) channels, cyclooxygenase and cyclooxygenase-1 with glibenclamide, indomethacin and SC560, respectively. Inhibition of vanilloid, transient receptor potential A1, transient receptor potential vanilloid 1, vasoactive intestinal peptide/pituitary adenylyl cyclase-activating polypeptide and calcitonin gene-related peptide receptors with capsazepine, HC030031, AMG9810, PACAP6-38 and CGRP8-37, respectively, also decreased electrical field stimulation and GYY4137 responses. H2S relaxation was not changed by guanylyl cyclase, protein kinase A, or Ca(2+) activated or voltage gated K(+) channel inhibitors. GYY4137 inhibited the contractions induced by phenylephrine and by K(+) enriched (80 mM) physiological saline solution. To a lesser extent it decreased the phenylephrine and K(+) induced increases in intracellular Ca(2+).H2S produces pig bladder neck relaxation via activation of adenosine 5'-triphosphate dependent K(+) channel and by smooth muscle intracellular Ca(2+) desensitization dependent mechanisms. H2S also promotes the release of sensory neuropeptides and cyclooxygenase-1 pathway derived prostanoids from capsaicin sensitive primary afferents via transient receptor potential A1, transient receptor potential vanilloid 1 and/or related ion channel activation.
- Subjects :
- medicine.medical_specialty
Sensory Receptor Cells
Swine
Calcitonin Gene-Related Peptide
Morpholines
Urology
Indomethacin
Urinary Bladder
Vasoactive intestinal peptide
Pig bladder
TRPV1
Calcitonin gene-related peptide
Neurotransmission
Synaptic Transmission
TRPV
Phenylephrine
Transient receptor potential channel
KATP Channels
Internal medicine
Glyburide
medicine
Animals
Calcium Signaling
Hydrogen Sulfide
Acrylamides
business.industry
Vasoactive intestinal peptide receptor
Muscle, Smooth
Organothiophosphorus Compounds
Bridged Bicyclo Compounds, Heterocyclic
Cyclic AMP-Dependent Protein Kinases
Electric Stimulation
Endocrinology
Guanylate Cyclase
Purines
Pituitary Adenylate Cyclase-Activating Polypeptide
Pyrazoles
Acetanilides
Capsaicin
business
Subjects
Details
- ISSN :
- 15273792 and 00225347
- Volume :
- 190
- Database :
- OpenAIRE
- Journal :
- Journal of Urology
- Accession number :
- edsair.doi.dedup.....e5949003043effe62454ff627c91e15d