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Real-time Functional Analysis of Inertial Microfluidic Devices via Spectral Domain Optical Coherence Tomography
- Source :
- Scientific Reports
- Publication Year :
- 2016
-
Abstract
- We report the application of spectral-domain optical coherence tomography (SD-OCT) technology that enables real-time functional analysis of sorting microparticles and cells in an inertial microfluidic device. We demonstrated high-speed, high-resolution acquisition of cross-sectional images at a frame rate of 350 Hz, with a lateral resolution of 3 μm and an axial resolution of 1 μm within the microfluidic channel filled with water. We analyzed the temporal sequence of cross-sectional SD-OCT images to determine the position and diameter of microspheres in a spiral microfluidic channel under various flow rates. We used microspheres with known diameters to validate the sub-micrometer precision of the particle size analysis based on a scattering model of spherical microparticles. An additional investigation of sorting live HT-29 cells in the spiral microfluidic channel indicated that the distribution of cells within in the microchannel has a close correspondence with the cells’ size distribution. The label-free real-time imaging and analysis of microscale particles in flow offers robustness for practical applications with live cells and allows us to better understand the mechanisms of particle separations in microfluidic sorting systems.
- Subjects :
- Materials science
Microfluidics
Cell Count
02 engineering and technology
01 natural sciences
Article
010309 optics
Optics
Optical coherence tomography
Lab-On-A-Chip Devices
0103 physical sciences
medicine
Humans
Microscale chemistry
Multidisciplinary
Microchannel
medicine.diagnostic_test
business.industry
Sorting
021001 nanoscience & nanotechnology
Frame rate
Flow Cytometry
Microspheres
Particle
Tomography
0210 nano-technology
business
HT29 Cells
Tomography, Optical Coherence
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....e59ba6d88fe03bce6ef4c4eeecc6acae